FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_G05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_G05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975116
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2750.071.218361
CGCACTA800.002047910636.2090848
GCCGACC1458.687657E-735.00538145
GGCCGTC1402.7678787E-531.0363567
GTATTAC5250.027.6332261
GTATATA10250.027.5995271
TATAAGG5000.027.549992
GCCGTCC1400.001006049125.863638
CGAAAGC9200.025.1979753
TACTGCG1450.00122642324.999992
CTAACCG2051.302598E-524.7256166
ACTACGA1801.5186614E-424.17038145
TATAGAC3301.7716957E-924.1666582
CTAGACA1500.001505004124.1369134
CTATAAG3702.582965E-1023.5255851
CTTACAC4455.456968E-1222.7832439
CTTATGC1600.00218486922.6388053
TAATACC3304.7512003E-821.9426484
GATCTAA5300.021.8980271
TATATAC11450.021.5118063
Produced by FastQC (version 0.11.5)