FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_G04-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_G04-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1047871
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT11230.10716968023735746No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA11080.1057382063250152No Hit
ATATAAATCCTGGCCATGCAGACCTCAGTGCCAAGGAGGCACAGGATGTA10500.10020317386395844No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGC406.6560155E-572.51608145
AGCGAAA1450.070.05571
ATATTCA3600.040.240564
GTATACC3700.039.149249
TAATGTA5150.036.561145
CTAGTAC2201.6370905E-1136.219963
GGTCGTT1458.7718945E-734.9626547
GTATTAA5550.033.990991
GTATTAC3650.033.793991
CTGTTCG1301.6664473E-533.4274259
GGTATAG1601.8922328E-631.6864159
GTACTGA3050.030.8671936
GGATAGC3450.029.3874935
GTAGAAA8200.029.2000431
GTATAGG3301.8189894E-1228.5301287
TACACTA1555.54263E-528.0332345
GTATAAT3403.6379788E-1227.7426451
AGGTATA1855.8649584E-627.4031588
ATATAGT5400.026.8732191
TAGTACT3005.9299055E-1026.558774
Produced by FastQC (version 0.11.5)