FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_G01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_G01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTATGGTTTAATTCCTCTGTTCCAGACAAGCAGGTCCCCTGAGAACA13.571428571428571No Hit
CATTGGAGGATTTTGATTATCAATCCACTTAGTAGTGTCCCAAGTGATTG13.571428571428571No Hit
ATAGAGGGCTAATCCATAAAAATAGTTCCTCCTCAATGGTGAAATTAATT13.571428571428571No Hit
CTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGAAAGAGGAATCCAGC13.571428571428571No Hit
CTTCTCAAAAAGTCCACCTCCAGAATGAACAAGATGATATTAAGCACAAG13.571428571428571No Hit
CTATATGTGTATCGGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGGG13.571428571428571No Hit
AATAAATGTACACAATGCAATTTCAAACATCAAATACCAGGAAGCTCCTA13.571428571428571No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG13.571428571428571No Hit
CAAATGTACTGCTGCTATTGGATAGAGCCATCGATATTTAATCCATTACA13.571428571428571No Hit
GGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTGTCTTGATCCGTC13.571428571428571No Hit
CTGTTGAACATATGGCAATAATCAAAAAGTAAACCTAAGGAATGCAAGAG13.571428571428571No Hit
ATGTAGGACCATCAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC13.571428571428571No Hit
GGGTCATGTCGGGCAGTATTCCGATCATTCCCATCACATTGTCGATGGAT13.571428571428571No Hit
ATCAAAATCACATTGAAACATGCAATCAAAGCGTCAATACTTATGAAAAC13.571428571428571No Hit
CCCATATATAGAAGAGTAGACAGAAAGTGGATGAGAGAACTTATCCTTTA13.571428571428571No Hit
GTGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCCCCATTACCT13.571428571428571No Hit
CTCAATGGTAATCGTGCGGGGGTTCTGCGCTTATGTTACCCTGTCAAGGA13.571428571428571No Hit
GGGGAAGGCGTTCCGTGATTAAAACCAGCCACTTCAATTTGAGTTGTCAT13.571428571428571No Hit
CTCTGGATCAGCTGTGTCATGGTATTAACCTTTGAAAGAATAATTTCATC13.571428571428571No Hit
AAAAATGTGTATAATAGGTTAATGATATCAATGATCATAGTTTTCTCAAC13.571428571428571No Hit
GATCAAAAGTACTTAAAAGGAAGAGGCAACACCCTTGGCCTCGATATCGA13.571428571428571No Hit
CTCTTTTTTATCATTAAATAAGCTTAAACGAGAAACATCTTATCTCTTTT13.571428571428571No Hit
GCTTGCTAGAGATCTGGGCTCTGGCTCATCACTGTCATACTCTTTAAGGT13.571428571428571No Hit
GTTCATTTATGATTAATGCTGGTACATATATCTTGTCTTCTTTGACAAAA13.571428571428571No Hit
AGCCGGTACAGAGCAATCGTAAGTACACTGTTCCAGCAAATGCGGGATGT13.571428571428571No Hit
ATCCTGGGCCATATCTCTTCTCTTCTTTGCCCAAAATGAGAAATCCTCTC13.571428571428571No Hit
GCAGAGCACAGGCGGGGATACGCTGTCGCTGAAAGTCAAGATGTGGCCAA13.571428571428571No Hit
CACAAATACAATAAAGAGGTCAAACTTAACGATAATGAAAAAAAATACAA13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)