FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_F12-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_F12-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13507
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTGCC1601.1845709632042645No Hit
TCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCCA1411.043903161323758No Hit
GGTCTAGACAGGACCCTGCTCTAAACTGGGGGCCCACAACTGGAAGCATG1401.0364995928037313No Hit
CGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAG750.5552676390019989No Hit
GCTCTAAACTGGGGGCCCACAACTGGAAGCATGTTGTCCCCCAACAATGA710.5256533649218923No Hit
ATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGG700.5182497964018656No Hit
GGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAGATCTATAACAGG700.5182497964018656No Hit
GATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGG660.48863552232175905No Hit
GATAAGGGGCGTCTGTCAGGGCACCTCAGACCTCAAAGACGAGCTGAAGC540.3997927000814393No Hit
GTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTCTAAGGCA530.3923891315614126No Hit
CCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACAC510.37758199452135927No Hit
GTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCAC510.37758199452135927No Hit
GTGATGGGGGATCTGCACGGGGACAGGACCCAGATCTATAACAGGGGATC480.35537128896127934No Hit
ACATAGCACCGTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCT470.3479677204412527No Hit
GTCCATAGCACTGGGGCTTGAGTTGCATCTGCCCTGCATCTGGGAGGTCG460.340564151921226No Hit
ACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAGATCTATA420.3109498778411194No Hit
AGTGAGGACCGCTCTCTGGCTGGCTGCCCGGGACGCGGGGCCTGGTTCCC410.3035463093210928No Hit
GCCTGGGGATAGAAGCCAGGCCTACAATGGGGGATCAGGTCTAGACAGGA380.2813356037610128No Hit
GTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTCTAAGGCAGGACG380.2813356037610128No Hit
AGCAGGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGG380.2813356037610128No Hit
CTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTGCCC370.2739320352409862No Hit
CTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGGGA370.2739320352409862No Hit
ATGTTTATTCACTCGAGAGAAAACACTTGCACATCACAGCTTAGCGTTCT350.2591248982009328No Hit
GGCCTACAATGGGGGATCAGGTCTAGACAGGACCCTGCTCTAAACTGGGG340.2517213296809062No Hit
AGACAGGACCCTGCTCTAAACTGGGGGCCCACAACTGGAAGCATGTTGTC340.2517213296809062No Hit
GGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGA340.2517213296809062No Hit
GCACGGGGACAGGACCCAGATCTATAACAGGGGATCTGCCTGGGGATAGA330.24431776116087953No Hit
ACCCAGATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACA330.24431776116087953No Hit
ATCTAAAATATCTCCCCATCCCATTACTCCCTGTCAAAGCCCTTTGTATC330.24431776116087953No Hit
GGACAGGACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAG320.2369141926408529No Hit
AGCAGGGACAGGACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGA310.22951062412082623No Hit
GCATCTGGGAGGTCGCGGAGAAACATCTTGAGTTGCAGGCATCTTTCTGA310.22951062412082623No Hit
CCATAGCACTGGGGCTTGAGTTGCATCTGCCCTGCATCTGGGAGGTCGCG300.2221070556007996No Hit
GTCCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTC300.2221070556007996No Hit
GGGCACACCTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCT300.2221070556007996No Hit
GGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGG280.20729991856074628No Hit
CTCATGGGCTACATGCACAATCTTCTCTTTCTTTCTTTCTTTCTTTTTTT280.20729991856074628No Hit
AATCCAATCAGGGCACACCTCTAAGGCAGGACGTCACCGAGTGAGGACCG270.19989635004071965No Hit
ATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGGGATC260.192492781520693No Hit
GTGTTAAATGATGGGGCTTTCTGGGCAGGCTGTTCTTGGGTGTGATCGAG260.192492781520693No Hit
AAGCAGGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTG260.192492781520693No Hit
CTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTATCCTGA260.192492781520693No Hit
GGACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAGATCTA260.192492781520693No Hit
GTCTAGACCTGATCCCCCATTGTAGGCCTGGCTTCTATCCCCAGGCAGAT260.192492781520693No Hit
GCCTACAATGGGGGATCAGGTCTAGACAGGACCCTGCTCTAAACTGGGGG260.192492781520693No Hit
AGCGAAAGCAGGGCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACA260.192492781520693No Hit
GTCCCCCAACAATGAAGGCAGTCAGATCTGGGGCCAGGGACCCCTGGGAA250.18508921300066633No Hit
ACAATGGGGGATCAGGTCTAGACAGGACCCTGCTCTAAACTGGGGGCCCA250.18508921300066633No Hit
CCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTAC250.18508921300066633No Hit
ATATCAGGCAGTCCCAGGACAAGAAGCCCGAGGAACCCACCAGCTCAGGT250.18508921300066633No Hit
CCACTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTCTA250.18508921300066633No Hit
GGATAGAAGCCAGGCCTACAATGGGGGATCAGGTCTAGACAGGACCCTGC250.18508921300066633No Hit
GTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGAC240.17768564448063967No Hit
CAACTGGAAGCATGTTGTCCCCCAACAATGAAGGCAGTCAGATCTGGGGC240.17768564448063967No Hit
GTTCAGGAAGGAGGGAATGGATCACATAGCACCGTGTTCAACCTTGAGAA240.17768564448063967No Hit
ATGCTACACGATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTC230.170282075960613No Hit
TTTCTATGTTCATTGGCTTTTGGTCCATCTTCCCTACTAAGCTGTAAGCT230.170282075960613No Hit
AATCAGGGCACACCTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTC220.16287850744058635No Hit
CATTTTGGGGGGAAATCCAATCAGGGCACACCTCTAAGGCAGGACGTCAC210.1554749389205597No Hit
GTCCTAGATTGGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATG210.1554749389205597No Hit
CCCAAGAACAGCCTGCCCAGAAAGCCCCATCATTTAACACCTAGGCGCCC210.1554749389205597No Hit
GCATTTGACATATAGAAAAGCACTTAGGGAAAATCATTGTGTGACTAACT200.14807137040053306No Hit
ATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAAATCC200.14807137040053306No Hit
TCCCATTACTCCCTGTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTT200.14807137040053306No Hit
GAAAGGGGGTGTCAGAGGGGAATGCCAGGTTTCTCTGACCCCAAAACTAG190.1406678018805064No Hit
AAAGCAGGCAGGTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCAGGAG190.1406678018805064No Hit
GATAACCCAGAGTCCATCTGAAAGCCACCTGAGCCAGCCTCTCGATCACA190.1406678018805064No Hit
ATTGGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATGCCAGGTT190.1406678018805064No Hit
CTCTCTGGCTGGCTGCCCGGGACGCGGGGCCTGGTTCCCAGGGGTCCCTG180.13326423336047974No Hit
GCCCATCCCACGCCTGTCCTGTCCACTGTTCAGTTACATTTTGGGGGGAA180.13326423336047974No Hit
ATATTAGATCACCCATTCCTACTGCAGACCTATCAAACAATCTTCTGCTA180.13326423336047974No Hit
TCTTAACACTTTTGTTACAAGCTGTAATCATTTTACTGATTTCTATGTTC180.13326423336047974No Hit
GTTTAGAGCAGGGTCCTGTCTAGACCTGATCCCCCATTGTAGGCCTGGCT180.13326423336047974No Hit
ATCATTGTGTGACTAACTGAATGAATAAATCCAACTTTCACCTGTTTTCC180.13326423336047974No Hit
CCAGTGGACTGAGTCAGGAGAAATTTAAGAAGTAAAATCTGGTCCTAGAT180.13326423336047974No Hit
TTTGTATCCTGAACACCCAGCATCGTGTAGCATTTGACATATAGAAAAGC180.13326423336047974No Hit
GGATACAATGGGCAAAGAAAGGGGGTGTCAGAGGGGAATGCCAGGTTTCT180.13326423336047974No Hit
CTTCCCTACTAAGCTGTAAGCTTCATGAAAACAGGAACCAGATTTTGTAT170.1258606648404531No Hit
AGCGAAAGCAGGGACAGGACCCAGGTCTGTGATGGGGGATCTGCACGGGG170.1258606648404531No Hit
GTTCTTGGGTGTGATCGAGAGGCTGGCTCAGGTGGCTTTCAGATGGACTC170.1258606648404531No Hit
GGTTCCCAGGGGTCCCTGGCCCCAGATCTGACTGCCTTCATTGTTGGGGG170.1258606648404531No Hit
CCACCAAGGTGGTGATCAATCTGTCAGCCTGTTGTCTTCAGGGACCTGCC170.1258606648404531No Hit
GTTCCCAGGGGTCCCTGGCCCCAGATCTGACTGCCTTCATTGTTGGGGGA170.1258606648404531No Hit
GGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTGCCCGGGACGCGGG170.1258606648404531No Hit
TTCCTGAACTGGCAATCCAGATAAAGGGGGGCTGTGAAGCCTATGGAGGT170.1258606648404531No Hit
GTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCAGGAGAAATTTAAGAA170.1258606648404531No Hit
GGTGTTGGCTGGCAGTGGGACCAGTGGACTGAGTCAGGAGAAATTTAAGA170.1258606648404531No Hit
GATCTGACTGCCTTCATTGTTGGGGGACAACATGCTTCCAGTTGTGGGCC160.11845709632042645No Hit
GCCCTGCATCTGGGAGGTCGCGGAGAAACATCTTGAGTTGCAGGCATCTT160.11845709632042645No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCAAAGGCAAAGGCTTTAACCC160.11845709632042645No Hit
GGATCTGCCTGGGGATAGAAGCCAGGCCTACAATGGGGGATCAGGTCTAG160.11845709632042645No Hit
CATTAGCTGAGTGTGCCAGGGAGCGGGAACAGTGTGGCTTGGGCAATTCT160.11845709632042645No Hit
CCTCCATTGCCACCACACCCCTCACCTCCATAGGCTTCACAGCCCCCCTT160.11845709632042645No Hit
GATCTGGGGCCAGGGACCCCTGGGAACCAGGCCCCGCGTCCCGGGCAGCC160.11845709632042645No Hit
GAATAAACATTGAGTGACTAAACACCACCAAGGTGGTGATCAATCTGTCA160.11845709632042645No Hit
CCTCCATAGGCTTCACAGCCCCCCTTTATCTGGATTGCCAGTTCAGGAAG160.11845709632042645No Hit
GTAAAATCTGGTCCTAGATTGGATACAATGGGCAAAGAAAGGGGGTGTCA160.11845709632042645No Hit
GTGTTCAACCTTGAGAATTTCTGGTAACAACTCATGGGCTACATGCACAA160.11845709632042645No Hit
CTGTTCAGTTACATTTTGGGGGGAAATCCAATCAGGGCACACCTCTAAGG160.11845709632042645No Hit
CCCTTGAGGTTTAGGGGTGGGGGAGGGTCTCGGTCCATAGCACTGGGGCT150.1110535278003998No Hit
GACAGGACCCAGGTCTGTGATGGGGGATCTGCACGGGGACAGGACCCAGA150.1110535278003998No Hit
GTGTTTAGTCACTCAATGTTTATTCACTCGAGAGAAAACACTTGCACATC150.1110535278003998No Hit
ACCCAGAGTCCATCTGAAAGCCACCTGAGCCAGCCTCTCGATCACACCCA150.1110535278003998No Hit
GTTTAGGGGTGGGGGAGGGTCTCGGTCCATAGCACTGGGGCTTGAGTTGC150.1110535278003998No Hit
GATCTGCACGGGGACAGGACCCAGATCTATAACAGGGGATCTGCCTGGGG150.1110535278003998No Hit
GGACCCAGATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAGGCCTA150.1110535278003998No Hit
GGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTGCCCGGGACG150.1110535278003998No Hit
ATGTTGTCCCCCAACAATGAAGGCAGTCAGATCTGGGGCCAGGGACCCCT150.1110535278003998No Hit
GCACTGGGGCTTGAGTTGCATCTGCCCTGCATCTGGGAGGTCGCGGAGAA150.1110535278003998No Hit
GTGCTATGTGATCCATTCCCTCCTTCCTGAACTGGCAATCCAGATAAAGG140.10364995928037314No Hit
CAACTCATGGGCTACATGCACAATCTTCTCTTTCTTTCTTTCTTTCTTTT140.10364995928037314No Hit
CTTCAGGGACCTGCCATTTAGGAAAACAGGTGAAAGTTGGATTTATTCAT140.10364995928037314No Hit
GGATTAGGTAGGAAGAGACTTGGAACATAGAAACTTAAAGAAAATCTTGG140.10364995928037314No Hit
GTAATCATTTTACTGATTTCTATGTTCATTGGCTTTTGGTCCATCTTCCC140.10364995928037314No Hit
AGCCAGGCCTACAATGGGGGATCAGGTCTAGACAGGACCCTGCTCTAAAC140.10364995928037314No Hit
ACTAAACACCACCAAGGTGGTGATCAATCTGTCAGCCTGTTGTCTTCAGG140.10364995928037314No Hit
CATCAGAATAGTGTGAAAGATTCTCATTAGTTTATGAGTGTAGGAGATCA140.10364995928037314No Hit
GTCAAAGCCCTTTGTATCCTTCTTAACACTTTTGTTACAAGCTGTAATCA140.10364995928037314No Hit
CACCTCTAAGGCAGGACGTCACCGAGTGAGGACCGCTCTCTGGCTGGCTG140.10364995928037314No Hit
CTGATAACCCAGAGTCCATCTGAAAGCCACCTGAGCCAGCCTCTCGATCA140.10364995928037314No Hit
ACCCAGCATCGTGTAGCATTTGACATATAGAAAAGCACTTAGGGAAAATC140.10364995928037314No Hit
ACTGGAAGCATGTTGTCCCCCAACAATGAAGGCAGTCAGATCTGGGGCCA140.10364995928037314No Hit
CCTTTATCTGGATTGCCAGTTCAGGAAGGAGGGAATGGATCACATAGCAC140.10364995928037314No Hit
GGACAGGACCCAGATCTATAACAGGGGATCTGCCTGGGGATAGAAGCCAG140.10364995928037314No Hit
ATGCTGGGTGTTCAGGATACAAAATCTGGTTCCTGTTTTCATGAAGCTTA140.10364995928037314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG100.006997186145.115232
CGAAAGC100.006997186145.115233
AGCGAAA100.006997186145.115231
ACACGGT203.7708294E-4108.836426145
ACTCGGT657.0528535E-444.65084145
CTAGACA1401.2778401E-841.3079384
GGTCTAG1451.6851118E-840.031791
TAGACAG1451.7420462E-839.8835265
TCTAGAC1655.2634277E-835.179453
GTCTAGA1655.2634277E-835.179452
ACAGGAC2151.5925252E-830.2604668
CAGGACC2353.783134E-827.6851069
GACAGGA2506.897608E-826.0247
AGACAGG2351.2060991E-624.6089846
GGGGGGG7000.012.438449145
GCGGTCC850.003291684411.950667135-139
GTGTGTG6152.571415E-511.7979881
TGTGTGT6505.8014574E-410.046442
GAACCAG1650.00567616247.9153767100-104
TTTTTTT2350.00242230186.792628385-89
Produced by FastQC (version 0.11.5)