FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_F05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_F05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences923450
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC11930.12918945259624237No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA10470.11337917591640045No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2500.049.3388751
TATACAG4900.039.9155275
GCGAAAG4000.030.8150672
GTATTAC9750.030.5112231
CGAAAGC4300.028.6480983
GTATATA7200.028.2166771
GTATAGG2705.515176E-926.8730261
ATAGTAC3800.026.6968353
TATTACA11450.025.9628642
ATTACAT11550.025.7227383
TACGCAG2559.5617E-825.5668095
TTACATC11200.024.5802784
ATAGACC6000.022.9465663
TGTAGCG1650.002613389421.9513025
TACATCA12300.021.7906825
GTATATG4409.640644E-1121.4373461
TAGACCT7550.021.1104244
TTAGCGT1750.003674014720.6991834
ACATCAA11950.020.6092266
TAGGCTT4952.1827873E-1120.487885
Produced by FastQC (version 0.11.5)