FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_F03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_F03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1043078
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG18010.17266206362323816No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT14080.1349851113723039No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC13870.13297183911462038No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA13640.13076682664191938No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT12410.11897480341834456No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11340.10871670191490952No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT10500.1006636128841755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAAA10350.056.055511
GGTCGTT4900.053.212367
AGGTCGT4900.053.2098126
AGCGAAA2150.050.596621
TCGTTTT6450.040.4248929
AGTAGAA7800.039.9799771
CAATTCG7100.039.784369
CGAAAGC3150.039.1162262
TATATTC8850.037.6533783
ATATTCA9900.036.5850264
TAGAAAC15350.036.358082
CCTTATA3900.035.31082
GCGCACG1905.4187694E-934.347492145
GACAGTA3700.033.27777
TTTAAAC13900.032.3121833
ACAAGGT11600.031.2249263
TTATACT5150.030.9445384
GGATAGC4350.029.9687425
ACAATTC10300.029.533728
CTGATCG2502.4429028E-929.00455145
Produced by FastQC (version 0.11.5)