FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_E11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_E11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences308647
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA8500.2753955165609904No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT8390.2718315745819658No Hit
GAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAACCTT7900.2559558330390381No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC7190.23295220753806128No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG6200.20087672972684004No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC6020.19504482467025436No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA5900.19115688796586391No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCATTTCATCAATGAGCA5650.18305701983171713No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC5520.1788450884019608No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC5460.17690112004976558No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT5420.1756051411483021No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG5280.17106921499317992No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA5040.163293341584399No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC5030.16296934685903314No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA4910.15908141015464267No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT4770.15454548399952048No Hit
CCTTTGGGTGACTCTCCAATGGGCCATGTTTCTGATCTATTCTCAAAAAA4700.1522775209219594No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA4390.1422336844356174No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA4130.13380982157610474No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA4060.13154185849854363No Hit
GGCATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC4000.1295978901463484No Hit
GGCAATGAAAGAGTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTG3980.12894990069561668No Hit
GTTCCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT3980.12894990069561668No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC3960.12830191124488494No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG3910.12668193761805557No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC3910.12668193761805557No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC3810.12344199036439688No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA3790.12279400091366513No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG3770.12214601146293337No Hit
GAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGT3760.12182201673756751No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA3710.12020204311073816No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT3710.12020204311073816No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA3690.11955405366000642No Hit
ATTCAATAGTCTGTATGCATCTCCACAATTAGAAGGATTTTCAGCTGAAT3610.11696209585707945No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG3460.11210217497659139No Hit
GTCCATATTATTTGGATCCCCATTCCCATTAAGGGCATTTTGGACAAAGC3430.11113019080049376No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC3310.10724225409610331No Hit
GTTGTAAACAGTATTTGCAACACCACAGGAGCTGAGAAACCAAAGTTTCT3280.1062702699200057No Hit
ATCTCAATGCATGTGTTAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA3260.10562228046927397No Hit
CCCTAGCTCCAGCGCTGGTCTGAAAAATGATCTTCTTGAAAATTTGCAGG3250.1052982857439081No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG3240.10497429101854222No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC3190.10335431739171286No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCCAGATTGTCCCT3150.10205833849024938No Hit
GATTTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC3130.10141034903951765No Hit
CTCCAATGGGCCATGTTTCTGATCTATTCTCAAAAAACTCTTTTGTCATG3100.10043836486342002No Hit
ATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACA3100.10043836486342002No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTAGA151.2283385E-4145.025131
GGTGACC100.0070848293145.02513145
GTACCGA100.007088265145.001622
CTGCTTA259.374092E-487.015076145
CATAGAA259.380133E-487.000972
GCCGACC353.43055E-582.871506145
TTAGTAG451.2295895E-680.569521
TAGATAT755.638867E-1177.284073
CTTATAA300.001933138972.512566145
CCATAGA300.001933138972.5125661
ATATTCA406.671577E-572.465564
AACACTG502.3089651E-672.465565
ATAGAAT300.001939365472.453833
GTAGAAA2250.070.901181
GTAGATA851.6916601E-1068.236052
GTACTGA1009.094947E-1265.219016
TCCCAAC606.7874953E-660.427141
GTAGTAT1102.5465852E-1159.3284651
TACTGAT1102.5465852E-1159.290017
CAAGGGT1450.054.973884
Produced by FastQC (version 0.11.5)