FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_E09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_E09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCCCAGATGGCTCTTCAATTGTTCATCAAAGACTACAGATACACATATA22.3255813953488373No Hit
AATCGAAACTAACAAGTTTGCTGCAATATGCACACATTTGGAAGTTTGTT11.1627906976744187No Hit
AAATAGAAGACTAGACAGAAAGTGGATGAAAGAACTTATCCATTAAGACA11.1627906976744187No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTAGTTTAATTACCAACACAACGT11.1627906976744187No Hit
TCCTAAATCTGATAAACAATAGACACTCCAAAAACAAGATCAAAACAGGC11.1627906976744187No Hit
TCCTGGATGCATCCCACCCAGGAATATTTGAGAATTCATGCCTTGAAGCT11.1627906976744187No Hit
TGTTTCAACATTTCGAACTTTTCTAAATAAGTTTTATATACCTTAGGGTA11.1627906976744187No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGCTAGCCCTTCGTT11.1627906976744187No Hit
CATATAGGAACATGGGCCTCGCCACTTGGCCTATCGCAGTCCTCAAGAGC11.1627906976744187No Hit
TTTGGTTGCCTTGTTGTAATTGAATACTGGAGAATTGCCTCTTACCAGTA11.1627906976744187No Hit
GACTTGGTGTATTTCTTTTGTCCAAGATTAAGTATCGAGACTACCAAGCC11.1627906976744187No Hit
CAATTGGATGTTTGTTTCCTGTATTCGGATTTCCATTTCATCGACGAACG11.1627906976744187No Hit
TGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTC11.1627906976744187No Hit
CTCTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAGCGT11.1627906976744187No Hit
CAATATACTGGTGGGATGGGCTCCAATCATCCGACGATTTTGCTCTCATA11.1627906976744187No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTTCGTTTAAATAACCACACAACGT11.1627906976744187No Hit
GGTAATTGGGATTTATGCCTTTCTCATGTGGTTTGACTATGTTAGGCTCT11.1627906976744187No Hit
CTAGTGGTCGCTGTATGCCTGTCAGTAATTATGCTAGAGTACCGTGTTAG11.1627906976744187No Hit
ACTGATGCCATGTCAACTGCAGCAATACCGTGAAACTACGGAGTCTAGTT11.1627906976744187No Hit
CAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCT11.1627906976744187No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA11.1627906976744187No Hit
TTATACTTGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCA11.1627906976744187No Hit
CATTCACATCTTTTCATTCCCTGGAGAGTAGATGGCCCCCAAAGCGGACT11.1627906976744187No Hit
GAACTGGGGCATGGAAATGAGGCGCTGCCTTCTTCAGTCTCTTCAGCAGA11.1627906976744187No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT11.1627906976744187No Hit
TATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTTGCAG11.1627906976744187No Hit
ATTCTGTTTACATTCATGATAAGATAATTACTGTTAGTAGCCATCTCTCT11.1627906976744187No Hit
ATCGGAGAGTCACAAAATGGAGTTGATGAAGGATCTATTTGGAAAGTGTG11.1627906976744187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1627906976744187No Hit
TCCCAGTTCCTGATTATCCATTGATAAGTGTTGACTAGCACTGACTCAGG11.1627906976744187No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT11.1627906976744187No Hit
GTGAATTGACTGATTCAAGCTGGATAGACCTTGATGAAATAGGAGAAGAT11.1627906976744187No Hit
GTGAGAATACCATGGCCACAATTATTGCATCAGCAATTGACTGCTCGTCT11.1627906976744187No Hit
GGAAGACTTTGTGCGCCAATGCTTCAATCCAATGATCGTCGAGCTTGCGG11.1627906976744187No Hit
GTCTATGGGATTCCTTTCGTCAGTCCGAAAGAGGCGAAGAGACAATTGAA11.1627906976744187No Hit
GTACTTAGCATGTTGAACATGCCCATCATCATCCCAGCACTCAGTGATGC11.1627906976744187No Hit
CCCCAAGATCGAAGGTTCCAGGTTCCAGGATGTCCCTAAGTGACTGAACA11.1627906976744187No Hit
CAATTGAAGTGGGCACTCGGTGAAAATATGGCACCAGAAAAAGTAGACTT11.1627906976744187No Hit
CTGCTAGCGAACAGCATGATAAACAATGACCTTGGACCTGCCACGGCCCA11.1627906976744187No Hit
ATCGTATTTTCAGGGTTTGGATGTTGCCGGGTAGCACTTCGTGTTCTTTG11.1627906976744187No Hit
CTCTCTGCATCTTTGGTCATCGTTTTCAATGTCAGTGCTCTTATTAGCTA11.1627906976744187No Hit
AGTAGAATCAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11.1627906976744187No Hit
CCTTGGCACTTAGCTCTGCATTGACATGGTTTGTATCATCTCTCGTGATT11.1627906976744187No Hit
GGAAAAGAGGCAGCAAAATGGGAGTAGATGAATACTCCAGCACGGAGAGA11.1627906976744187No Hit
AAGGGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTC11.1627906976744187No Hit
GTATGCTAGAGTCCCGGTTTTGGTTGATTTCAAACACAAAGTACCCTTGC11.1627906976744187No Hit
CACATGATGTGATAGCTGCAAGCACTGCAGAGGTTGAGATGTCAATCCTG11.1627906976744187No Hit
CTCTTGGAAATGTGCGACAGACAAAAGATTGCAGGAGTAAGGATGGTGGA11.1627906976744187No Hit
GTATGAGGCTGCATGACCAAGATGCTGGTCCCGCAAGGCTCAATAGGGAA11.1627906976744187No Hit
GTGCTGGGTAGGACCCTAAGCACACCGGAGGAGCCATATATAGAAGAGTA11.1627906976744187No Hit
AACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAA11.1627906976744187No Hit
GCATAAATCCCAATTACCTCATGGCTTGGAAGCAGGTGCTAGCAGAGCTA11.1627906976744187No Hit
GCCATAGGGGAGCGACAACAGTTCAGACGAGATGATCATTTGAGTTACAG11.1627906976744187No Hit
GGAATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTGTTCAACAAAC11.1627906976744187No Hit
ACTTAGAATATATCTTCCTCTTCTCCCAACCATTATGATTTATACATACC11.1627906976744187No Hit
AAGTAAGAGAAAGTCGAAACCCAGGAAACGCTGAGATTGAAGCCCTCATT11.1627906976744187No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11.1627906976744187No Hit
TGTGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCGTC11.1627906976744187No Hit
AAACAAGGTCGTTTTTAAACAATTCGCCACTAATTGAGGGCCATCCGGAT11.1627906976744187No Hit
GCTCTGGCTCATCACTGTCATACTGTTTAAGGTCTCCAACATCTTTGCAG11.1627906976744187No Hit
GACCTGAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAG11.1627906976744187No Hit
GCCATGGAACAGGAACAGGATACCCCATGGACACAGTAAACAGACCACAC11.1627906976744187No Hit
ATTCCACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTT11.1627906976744187No Hit
GCCGCCCCAATGCTAGCAAATCCAACCGGTCTCCTATATCAACTGCTAGG11.1627906976744187No Hit
CACCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATG11.1627906976744187No Hit
GTACATCTGTTCATCCTCAAGAATTCACCATTGGCTTGTGTTTAGAATAG11.1627906976744187No Hit
GAGCATTTGCGAAAAGCTTGAACAGTCTGGGCTCCCAGTAGGGGGCAATG11.1627906976744187No Hit
TATATACCCTTGTTTGTTGCACACCTTCCATTGTTTCAAGGCATGAATTC11.1627906976744187No Hit
AAATACGGCCTAGCACAATGAATCCTGTGCAGCCATGGATGCCTATCAGC11.1627906976744187No Hit
TCATCATCCCCGGACTCAGTGATGCTGTGCGAGCTATTAGAAGAGGCCGT11.1627906976744187No Hit
TATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTT11.1627906976744187No Hit
GCGTAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA11.1627906976744187No Hit
GAGAATAGCTGTTGCTCTTCTCCCAACCATTGTGAATTCTTCATACCCTT11.1627906976744187No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATTATGTGGATGATG11.1627906976744187No Hit
CATTTGAATTCACAAGCTTTTTTTATCGCTATGGATTTGTGGCTAATTTT11.1627906976744187No Hit
ACCTAGCGAAAGCCAACAAAATAAAATCTGAGCAGACACACATTCACATC11.1627906976744187No Hit
GGGGTGGAGTCTGCTGTCCTGAGAGGATTTCTCATTTTTTTCAAAGAAGA11.1627906976744187No Hit
ATTCCACTCTAATTGATGGCGATCCCTATTCTATTGGTCGCTGTCTGGCT11.1627906976744187No Hit
GTGCCTGAACAATGAGAAGCAATTTTCTAGATTAATCCGAAAACCCCTCA11.1627906976744187No Hit
CTCCAAAACTCATGTCAGCTGATAGAATTACTCCAGACACTCAAAAGCTG11.1627906976744187No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA11.1627906976744187No Hit
CAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAA11.1627906976744187No Hit
ACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGA11.1627906976744187No Hit
TCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACAACCCCTGGCCAG11.1627906976744187No Hit
AATATACAATATACGGAATCTACACATTCCTGAAGTCTGCATAAAATGGG11.1627906976744187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAATGT50.0145.07
CTCTTGG50.0145.01
CTTGGAA50.0145.03
TTGACTT50.0145.0145
TCTTGGA50.0145.02
TGGAAAT50.0145.05
TTGGAAA50.0145.04
AATGTGC50.0145.09
AAATGTG50.0145.08
GGAAATG50.0145.06
Produced by FastQC (version 0.11.5)