FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_E07-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_E07-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828163
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2650.049.271681
CGAAAGC3500.037.2583773
GCGAAAG4150.033.184472
TATACAG6500.032.3185355
GTATATA12550.027.7439441
CAGCTCG6450.025.8290799
GTATTAG2859.833457E-925.452231
GTAATAC8300.025.3127543
ATATATG6900.024.1489493
TAGATAG3601.8553692E-1024.1460325
TACCTAT7500.024.1431147
TATAGAG2751.9817526E-723.7098773
TATACTC2151.8776032E-523.5844945
TAATACC8350.023.4216844
TATATAT8700.023.32752
GATGCGT2802.3609937E-723.2836726
TATAGAC6700.022.7181992
AATACCT7900.022.0065125
TATATAC13550.021.9211863
TATATGT7950.021.8667834
Produced by FastQC (version 0.11.5)