FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_E05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_E05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAATTTTGCAGTCTGAGTTCTTCAATGGTGGAAAAGATCTTCATGAT17.6923076923076925No Hit
GAGGAATAAATATCTGGAAGAACATCACAGCCCGGGGAAAGATCCTAAGA17.6923076923076925No Hit
CATTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCAAAGCGAGA17.6923076923076925No Hit
CAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGACAAAG17.6923076923076925No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC17.6923076923076925No Hit
ATGTTGGAGCCCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGCTCT17.6923076923076925No Hit
CCTAGGACTAAGGTTATTTTAGGATTTGTGTTCACGCTAACAGTCACCAG17.6923076923076925No Hit
CTGTTAGGCCCGTCGCTCTAAAGCCTGCTAGGGTGTTGGCCCATGCAGTT17.6923076923076925No Hit
CATGTTGGAGAGAGAACTGGTCCGCAAAACGAGATTCCTCCCAGTGGCTG17.6923076923076925No Hit
AACTAATCCAAGTGTTTCAGATATTACCAGTTTCAAAAATTACACATATT17.6923076923076925No Hit
GTCCCCAGTGATTGGGAAAGAAATCTCTGTGTCTTGTGAAGTAGTCATCA17.6923076923076925No Hit
GCCTGCTCTAGGTACAAGGCCAGCTCAGAGTCGGATGGATACCATGGAAG17.6923076923076925No Hit
CTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAAT17.6923076923076925No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)