FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_E01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_E01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTAGGATGGTAGAAATCATTATTCAGAAAACAACATAAGATAAAGAAT12.857142857142857No Hit
ATTTGTTCTAGTTCTGTGAGAAGTGTTATTGGTATTTTGATAGGTATTAC12.857142857142857No Hit
TCTCTTGTCTTCTTTGCGCAGAATGATGAATCCCCACAGCACAGCGGACT12.857142857142857No Hit
ATCCACGGCTTGCTCTTCTGTTGGGTACTGCCTAAGGATGACTACCATCC12.857142857142857No Hit
CAGACATTCTCAGACAGCAACCCAGGAGAATCAGCATGTTGAGTGTAATA12.857142857142857No Hit
TTGCAGCCTTGCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTA12.857142857142857No Hit
CAGGAATCTCGATTTGCGGACCAGTTATCTCTCCAACATGTATGCAACCA12.857142857142857No Hit
CTCAAAAAAAGGTCAATGATCACAACTACCATCTCCGTTATGGAGTACTC12.857142857142857No Hit
AAATATCAGAGAAAATTCAACATTCTAAAATGTAAAAATAAATCATCTTC12.857142857142857No Hit
ATGATGATCTAGAAAAAAACTAGATTATTGCTGCTAGCAACATAGTGAGA12.857142857142857No Hit
CCATCAATCCTTCAGATACTGCATCATTCATATTACTCCATAAAGTTTGT12.857142857142857No Hit
CCCTTGACCTCCTCCATGGGCGCTGGCCCCAAATAAGATACATAGAGCCC12.857142857142857No Hit
TGTGAGGGGATCAGGAATGAGAATACTTGTAAGTGTCAATAATAATGTAT12.857142857142857No Hit
TTGCCGAAGCAATTATTGTGGCCATGGTATTTTCACAAGAGGATTGTATG12.857142857142857No Hit
CAAGACCCCAGCAATTACGATTAAATGGATGATGGCAATGAAATATCCAA12.857142857142857No Hit
TTACTACATTTCCTCGATCGACCCGGATTCTAAAAAAACGGTCAATGCTA12.857142857142857No Hit
GTCGATAGGTTCAACTGCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT12.857142857142857No Hit
AAAAAGCACTGTCGCATCCTTATTAAAATGTCTAAAAAGTTGATGCAAAG12.857142857142857No Hit
GTGGAAAAGCAAAGTCCCCACACCAACTACACTTAATTACCCTAACCGAT12.857142857142857No Hit
CGCCGAGGGGGGGGGGGGGGGGGCGGTCGGGGAGCGGGGCGTCGTGGGGG12.857142857142857No Hit
GCTTGTTACACCATACACTGGGAGTCATATCGTTTTTCGGACCAGTGCTC12.857142857142857No Hit
GAGAAGAGCAACACCCATACAAATAAAAGCAACAAGGAGATACATTCAGC12.857142857142857No Hit
TCTTAAAAGTTGATGCATAGGATTCAATCGCTGATTCGCCCTATTGACGA12.857142857142857No Hit
CTCACTATCATCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGATGT12.857142857142857No Hit
ACCATAGGCAAATCCCAGCAGAAACGCATGCCATTGAAAAATCAAAGTAT12.857142857142857No Hit
CAGAACATCCGCCGTGGCCCAGATGGCCATAATCAAGGTGTAGCCCTGAG12.857142857142857No Hit
GTAGTGGTGAGCATTGCCCGTTTTTTGATAATCCGGGACCAACGAGGATA12.857142857142857No Hit
CTACAATAAAATGTAATTTGAACCATGTCAGTATTTAGTACCTAAGTCCA12.857142857142857No Hit
GCATCAGTCAAGAGCGATGAAGCTGTGATTACGGGCAATCGTCAAAAATT12.857142857142857No Hit
GTTCATTGCTGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA12.857142857142857No Hit
TTGGAAAGTTGAAACTATCCACAATGTGATGCGAAAGATTCTGAAAATCA12.857142857142857No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT12.857142857142857No Hit
ATTAATGGCCCTGAGTCAGTATTGGTCAATACCTATCAGTGGATCATCAG12.857142857142857No Hit
GCTAATTAGTCAGGCAGGCTAATAGATTTCTTAAACGATGTAATGGAATC12.857142857142857No Hit
GTTCGGTAGTTTATGGGGAGCAAATTGGAATTTTACCCCCGAGGGATGCT12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)