FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_E01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_E01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329689
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG7750.23507002053450374No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA7470.22657716817970877No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC6690.20291850804849418No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC6390.19381902338264242No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG5940.1801697963838648No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA5670.17198026018459822No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC5370.16288077551874647No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA5100.15469123931947987No Hit
TCCCAACACTGTGTCAAGCTTTCAGGTAGATTGCTTCCTTTGGCATGTCC5080.1540846070084231No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA5070.1537812908528947No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA5000.15165807776419596No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT4900.14862491620891205No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT4640.14073869616517384No Hit
TCATTACTCATGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTC4390.13315579227696406No Hit
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC4220.1279994176329814No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG4190.1270894691663962No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG4030.12223641067794194No Hit
CCCTAGGAGATCTGGAGCTGCTGGCGCTGCAGTCAAAGGAGTTGGAACAA4010.12162977836688514No Hit
ATTCACAGCTGTGGGCAAAGAATTCAACAAATTAGAAAGAAGGATGGAAA3950.1198098814337148No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT3930.11920324912265802No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC3850.11677671987843088No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG3800.11526013910078893No Hit
ATATAATGCAGAATTGTTGGTTCTACTGGAAAATGAAAGGACTTTGGATT3780.11465350678973214No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAAAACGTTCTTA3750.11374355832314696No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC3720.11283360985656178No Hit
GTCATAAGTTCCATTTTTCACACTTTCCATGCATTCATTGTTACACTTGT3620.10980044830127787No Hit
ATCATAATGTGAGTCAAACCAGCCGTTGCATCATCACCATTGTTGGCTTG3400.10312749287965325No Hit
CTCTAATATTACTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA3380.10252086056859647No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC3370.10221754441306807No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG3370.10221754441306807No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT3340.10130759594648289No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACCG100.0070824595145.04283145
AGTACCG100.0070824595145.04283145
AGCGAAA203.849869E-4108.848271
GGCAGCC301.5955158E-596.69522145
TACTGAT451.0963049E-896.577857
GTACTGA451.0975782E-896.56326
TTAGTAG502.2497261E-887.078621
AAGGTAC353.4495402E-582.781029
GGGTAGA554.3579348E-879.162381
TACTAGT451.19093136E-464.463482
GTAGAAA2650.062.9813881
ACATAAT1102.5465852E-1159.335712
TACTCTA1102.5465852E-1159.2636877
TCGTACT2450.059.2011572
CGTACTC2450.059.138273
TTCAACG400.00606932954.3250478
TCAACGG400.00606932954.3250479
CTACTAG553.2015145E-452.7749181
AATTATA553.2111385E-452.742852
CAAGGGT1850.050.899148
Produced by FastQC (version 0.11.5)