FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_D10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_D10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences983792
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA56670.5760363979377754No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT45830.4658505049847935No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT33780.34336526420218905No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33370.3391977165904988No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG29750.30240132060435543No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA27350.2780059199505587No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT27290.2773960349342137No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA24010.24405565404069154No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC23280.23663538634182835No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC21580.21935531087872231No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG21240.21589929578610112No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG21210.2155943532779287No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC20900.21244328069347992No Hit
CAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGGACGACAAAC20320.20654772553547904No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG18900.1921137801486493No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG18800.19109730512140777No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG18630.18936929757509718No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG18380.18682811000699334No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT18010.18306715240619967No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC17240.1752402946964399No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG16740.17015791956023224No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT16060.16324588937498982No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA15820.16080634930961016No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA15640.15897669426057542No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG15230.15480914664888515No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC15150.15399596662709192No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT15100.15348772911347114No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT15030.15277619659440206No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG14750.1499300665181258No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT14670.14911688649633256No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG13930.14159497129474521No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG13710.13935872623481385No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG13560.13783401369395157No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA13100.13315822856864054No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC13000.13214175354139898No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA12620.12827914843788119No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG12350.12553466586432904No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA12060.12258688828532861No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCG11760.11953746320360402No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG11730.11923252069543155No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC11680.11872428318181079No Hit
CAATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA11600.11791110316001757No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT11500.11689462813277603No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG11240.11425179306194806No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA11130.11313367053198238No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA10780.10957600793663701No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA10680.10855953290939548No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC10510.10683152536308488No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG10420.10591669783856751No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG10350.10520516531949842No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG10350.10520516531949842No Hit
GCTTCATATAGCCCCCCAAGATCGAAGGTTCCAGGTTCCAGGTTGTCCCT9900.10063102769691154No Hit
CCCTTACACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA9880.10042773269146324No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACCTG203.872214E-4108.7063753
AGTAGAA7600.0105.9911961
GCAGGTA3200.099.7134252
CAGGTAC3300.096.6278843
ACTAGGA259.3770097E-487.022622
GAGACGT259.3789055E-487.01819145
CCGCACA259.3789055E-487.01819145
GACAGTA4900.084.268627
AGCGAAA2000.083.456121
CGAAAGC2200.082.353313
GGTACTG3900.081.732965
GCGAAAG2100.079.425412
AGGTACT4750.070.160954
GTAGAAA12200.065.385852
GAAAGCA3000.062.7889674
CCCTTAC2550.062.609891
CTACACT606.834074E-660.3740044
GATCTTA2000.058.0564351
CTTACAC2750.057.9767343
CCTTACA2900.055.01432
Produced by FastQC (version 0.11.5)