FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_D05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_D05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAGGGCCATGGCCCTGGAGAAGTTACTTAATTAACCTCTTTGAATGGG18.333333333333332No Hit
GTATCGCGACTCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATC18.333333333333332No Hit
GCATCCATCAGCAGGAACTTTGACCGCTGAAGGCAAAGAGGCCCATCAGG18.333333333333332No Hit
GATTGCAGCCCTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGG18.333333333333332No Hit
GAACATTATGGGTGCCATGCGCATGATTTTTCTGACACACTCGGGGTGAT18.333333333333332No Hit
CCTTTGCTGATGCCCCACCAGAACAGAGTAGGATGCAATTTTCCTCATTG18.333333333333332No Hit
CATGTAAACATGCAGTTTTAATTCTCACAGTGGCACTGTGAGGTAAGAAG18.333333333333332No Hit
GGCTGGGGGCTGGCTCGGGGCTGCGAGGCAGGCAGTGGGATCTTCTGGCA18.333333333333332No Hit
ACTGAAAGATGAGCTAATCCTCAAGCCTATTGCTCCCTTGCTTATGTCAA18.333333333333332No Hit
GTAATAAAGAGATTGAGTCTGTACACAATGCTGTGGTCATGCCCGCCCAT18.333333333333332No Hit
ACTTAGGGACAACCTGGAACCTGGAACCTTCGATCTTGGGGGGCTATATG18.333333333333332No Hit
CTTAATATTATTATTAGAAACCTCAATGTCCTAAGCTGCATGTTCACTCG18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)