FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_D05-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_D05-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997334
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG17510.17556806445984996No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA14670.14709214766567671No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12530.1256349427573912No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11360.11390366717669306No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC10630.10658415335283868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG6150.044.7661179
AGCGAAA2950.044.264761
CGAAAGC2800.043.9921723
GTATCCG951.0251341E-438.1815871
TATCCGA951.02816906E-438.1624262
AGTAGAA9600.032.494121
GCGAAAG3800.032.4380652
GTATAGG2351.2551027E-930.8702161
ACAATTC9100.030.2540258
TAGAAAC12050.030.0654353
GTAGAAA12100.029.977281
CCTTATA8050.029.7240262
GGTCAAA7500.029.0180071
AGGTCGT4500.028.9787186
TTTAAAC11950.027.8916663
GGTCGTT4500.027.3701617
GGATAGC3750.027.0468045
TATATTC8850.026.1993943
TATACTG11000.025.0270755
TTATACT10650.023.8076114
Produced by FastQC (version 0.11.5)