FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_D03-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_D03-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107894
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG34990.3158244380780111No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA33010.2979526922250685No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA22090.19938730600580923No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT20600.18593836594475646No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG18860.17023289231641295No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA17330.15642290688459365No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA14950.1349407073239859No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC12930.11670791610027674No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT12470.1125558943364618No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG11660.10524472557843982No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG11550.1042518508088319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTCG10550.056.2855229
ATTATAT13550.050.8632971
TCAATAT13150.049.562378
TATATTC14000.048.6486133
AGCGAAA2400.048.364751
TTATATT13850.046.5892332
TATTCAA16000.041.6430325
TTCAATA15350.041.5154347
ACTATTC15200.040.9722828
ATATTCA16050.040.616344
CAATATG16550.038.942819
TTAAACT17800.038.250864
TAAACTA15850.037.467885
ATTCAAT18000.036.610386
AAACTAT16850.036.1006246
AACTATT17950.033.888337
GGTCGTT5800.033.7109577
TTTAAAC19450.032.7818683
CGGGGTA1504.433992E-528.966459
GTATATA25900.028.8507861
Produced by FastQC (version 0.11.5)