FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_D01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_D01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAAATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTT12.272727272727273No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC12.272727272727273No Hit
GAAGAAAAAAGCTATGGTTATATCAGAAAATACGCGTAAACGACAGAGCT12.272727272727273No Hit
ATCAAGGACAAACCCTCTGCTAAAAAACAAACGATGCTGGATCAGACCCA12.272727272727273No Hit
CTCTCCTGTCTTGGGGTCATGTCGGGCCGGATTCCGATCATTCCCAGCAC12.272727272727273No Hit
CAACCAGTCCAGTTCCTTACCCTACGGTATATAACACTGATTTCGAAAAG12.272727272727273No Hit
CAAATGATCTTCTCGTCTGAATTTGTGTGTCTCCACTATGGCACCTATAT12.272727272727273No Hit
TAGTCAAACCACATGAGAAAGGCATAAATCCCAATTACCTCATGGCTTCC12.272727272727273No Hit
ATGCAAGAACTCTTGACGCATTTCCAAAAATATGCAAAACAGCTTATCCA12.272727272727273No Hit
ACCATGCCCTTGGACCTGCCACGGCACAGATGGCTCTTCAAGTGTTCATC12.272727272727273No Hit
GGCAATAGACACCATGCCCTCCAAAATCCTAGCAATTCCAACCGGTCTCC12.272727272727273No Hit
AGTTCAATCCAGAAAGAATCATTAACATCCCATGACTTAATCTAATAAAT12.272727272727273No Hit
CCAACACAACGTCCCCTACCCAAAATAGCAAATTAGACAACTCTCCATTT12.272727272727273No Hit
GAGTAAGGATGGTTGACATCCTTAGACAGAATCCTACTGAGGAACAAGCC12.272727272727273No Hit
CTTCTTGATACGTCCAGACTCGAAGTCGACCCTGGAATCAATCCGGGCCC12.272727272727273No Hit
CACTAAGACAACTGTTGAAAATATGGAAATAATAAAAAATTACACATCAT12.272727272727273No Hit
CCAATACTCATGTCCGCTGATTCAGTTACTCCAGCCACTCCAAAGCTGGG12.272727272727273No Hit
ATATATATAAGCTGAGCCGGAAATTTGATTAGAGCACTCATAATTTATGC12.272727272727273No Hit
ATATAAAACTGGAAATCATTAAAGTACAGACAGAATTTAAGGTCTTAGAA12.272727272727273No Hit
CATATCATGCATGTTGAAGAAGTGGTTGGGGTTAAACCTGTTGTGGTCCA12.272727272727273No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC12.272727272727273No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG12.272727272727273No Hit
CACCATGATCAAACCACTCTGTAATCAAATCAAAAAATATATTCAATGCT12.272727272727273No Hit
TTCAAGAAGTTAGAGTTTAAGAAAATTCAACCCTTTAGCTGAAGTGGAAA12.272727272727273No Hit
GGCTTCGCTCGGTTTGCAAGTGTGGTCAGTTCCTTGATGGTTACTGCTGG12.272727272727273No Hit
GTATTTCTTTTGTCCAAGATTCAGTATCGAGACTCCCAAGACCGTACTTC12.272727272727273No Hit
ATTCAGTAGCCCTGCAGTGGGACACTTCGGCTGTAAAATAGTTCCTCATC12.272727272727273No Hit
AAATATGGGTGCAATGATCAGGATCTATATGACCCACTAGAGTAGATTTC12.272727272727273No Hit
ACAAAGTTCAGATCGCCACTAACTGCCTTGATCATGCAATCCTCCTGTGA12.272727272727273No Hit
TCTCATCTCTTGTCTTCTTTGCCCAAAATGAGAAATCCTCTCAGGACAGC12.272727272727273No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTTCACACTTTC12.272727272727273No Hit
GTCGACCCCCTTGAACCATTAACACTCAACCAAACCTACGAAGAACATGT12.272727272727273No Hit
ACTCAGCACCGTCTGGCCAAGACCCACCCACAGTGGCACTGTTTACCCCA12.272727272727273No Hit
CTCCAAAGCAGGCAATAGAGATGATAAAAGAAGAAGCTTACCCATATCAA12.272727272727273No Hit
CCATAGAAGTCTTTAGATCGAATGGCCTAACAGCTAATGAGTCAGGAAGG12.272727272727273No Hit
TTAGCGTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAAGC12.272727272727273No Hit
GATGTAATTGAGAAACACATTGCAATCATACACTTCATTCTGAGAGCGCT12.272727272727273No Hit
AGACGGTTGATTACAGATTTTGGGAGTAAGGTCCTGCACACTTTGCCAAT12.272727272727273No Hit
CCACGTCTCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTCGCAACG12.272727272727273No Hit
GAAACAAGCTACTAATTAGCACAGTAGCGATATCAAATAGTAAAAATGCC12.272727272727273No Hit
AATCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC12.272727272727273No Hit
GAATTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATCCTCG12.272727272727273No Hit
GAGCCACACAAATGGGAAAAATACTGTGTTCTTGAAATAGGAGACATGCT12.272727272727273No Hit
CCCTAGACAACCTGGAATACAACATGCTAGAAATTCAAAACGGTCGCATA12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)