FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_D01-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_D01-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117155
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG19310.17284978360209638No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA18300.16380896115579308No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA13360.11958949295308172No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT12000.10741571223330693No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT11680.10455129324041874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3200.063.483621
GCGTAAA350.003556357762.1880381
CTATTCG6950.050.025359
CGAAAGC4300.047.1695943
TATACGT3050.037.99744
TATATTC11150.037.031613
CAATTCG2650.035.5329449
GGTCGTT4300.035.374037
TTAAACT11600.034.9674344
AGGTCGT4350.034.9674346
ATTATAT10550.033.6974681
GTAGAAA12550.033.5303341
CGTGCTA3100.032.711478
TTTAAAC15100.032.141823
AGTAGAA9350.031.8145581
GTCGTTT5250.031.732358
TCAATAT12200.031.4665978
ATATTCA12850.031.0022534
TAGACGT950.00472523430.529345145
ACTATTC12350.030.4979138
Produced by FastQC (version 0.11.5)