FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_C11_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_C11_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences727631
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG58480.8037040752799152No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA43240.5942572540202383No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA37710.5182571935500274No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA36790.5056134221878947No Hit
GTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCC35370.48609803595503764No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG28840.39635474574337815No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG27670.3802751669458833No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT26790.36818112477340853No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT25380.34880317083796597No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT23220.3191177945964369No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC22430.3082606431006925No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG21860.30042700214806684No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT21170.29094417362646724No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT19980.2745897302341434No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC18280.25122623967368074No Hit
AGTAGAAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATC17230.23679584844515972No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC17020.2339097701994555No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG15070.20711047220363069No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT14970.20573614922948583No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA14650.20133831571222224No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC13970.1919929194880372No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAA13620.18718278907853017No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG13430.18457157542765495No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA13320.1830598201560956No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG13300.18278495556126662No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC12970.17824968974658859No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC12950.1779748251517596No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG12610.1733021270396671No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA12490.17165293947069327No Hit
CTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGA12470.1713780748758643No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG12290.16890429352240355No Hit
ATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACT10980.15090066256110585No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA10950.15048836566886237No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT10890.14966377188437546No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG10790.1482894489102306No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC10760.14787715201798715No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA10540.14485364147486846No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA10380.14265472471623666No Hit
ATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTC10170.13976864647053244No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC10120.13908148498346No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA10120.13908148498346No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT10060.1382568911989731No Hit
GTTCATTGATGCTTAATGCTGGGCTCTTGTCTTCTTTGCCCAAAATGAGA10030.13784459430672963No Hit
ATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTA9940.13660770362999927No Hit
CATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAAT9890.13592054214292684No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA9830.1350959483584399No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG9700.1333093284920516No Hit
TTACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCT9520.13083554713859086No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG9510.13069811484117635No Hit
AATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCAC9390.12904892727220252No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG9300.12781203659547216No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG9290.12767460429805766No Hit
GATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGA9270.1273997397032287No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA9030.12410136456528104No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC8840.12149015091440579No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT8790.12080298942733336No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG8700.11956609875060299No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGGACACAGTAAACAG8570.11777947888421465No Hit
CCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTGG8500.11681745280231325No Hit
AATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGA8430.11585542672041187No Hit
GACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC8330.11448110374626701No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC8320.1143436714488525No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT8200.11269448387987867No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC8150.11200732239280624No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA8100.11132016090573381No Hit
GGATACACCATGGACACAGTAAACAGAACACACCAATACTCAGAAAAGGG8010.11008327022900344No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC7970.1095335410393455No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG7800.10719719198329922No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT7760.10664746279364129No Hit
GAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAA7680.10554800441432538No Hit
ATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCC7670.1054105721169109No Hit
GTATATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTG7530.10348651995310809No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG7530.10348651995310809No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA7480.10279935846603566No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT7430.10211219697896323No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGATA100.0070833545145.04868145
GTTAGAT100.0070848125145.038681
AGGTATG100.0071169427144.819355
AGTAGAA5450.0114.43421
GCAGGTA2700.0110.053782
CAGGTAC2850.0101.6485753
GGTACTG2900.099.875435
AGCGAAA1300.094.8329851
AACTCAC2000.094.139058
CGAAAGC1600.076.951153
AAACTCA2500.075.311247
AGGTACT4050.075.091524
GCGAAAG1650.074.670642
CCGCACG406.6508925E-572.52434145
GTAGAAA10000.068.168181
GACAGTA3500.066.207687
ACTGATC4350.064.9234858
AAATATA8550.064.364165
CTGATAA350.00356137262.16372145
TCAGTGG350.003580839662.078243
Produced by FastQC (version 0.11.5)