FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_C10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_C10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863820
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG21020.24333773239795328No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20810.24090667037114213No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA18110.20965015859785605No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA15790.1827927114445139No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT14280.1653122178231576No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC13950.16149197749531152No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG13520.15651408858326968No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG13340.15443032113171726No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG13070.15130466995438865No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA12810.1482947836354796No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG12460.14424301359079436No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG12170.1408858326966266No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT12080.1398439489708504No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT12050.13949665439559167No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG11950.13833900581139588No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG11790.13648676807668264No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT11780.13637100321826306No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA11670.1350975897756477No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC11600.13428723576671067No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11330.13116158458938204No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA11260.130351230580445No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC11220.12988817114676668No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA10820.12525757680998356No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT10810.12514181195156399No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT10550.12213192563265496No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT10300.1192378041721655No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG9820.11368109096802574No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG9770.11310226667592786No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT9420.10905049663124262No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT9360.10835590748072516No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT9300.10766131833020767No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT9070.10499872658655737No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA9050.10476719686971823No Hit
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA9000.10418837257762034No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA8800.10187307540922878No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGGCG100.0070842016145.04443145
AGTAGAA2950.083.584931
AGCGAAA1150.081.981631
GCGAAAG1150.081.929362
CGAAAGC1250.075.305173
GCTATAC300.001945340172.408813
TGGTTCG300.001948470572.379485
CAGGTAC902.8558134E-1064.3633963
CTACACT1250.063.7013174
CCGACGC808.065399E-963.45694145
GGTTCGG350.003588275562.0467346
GTAGAAA4100.061.9092061
TCTACAC1300.061.2689973
CTGCCGC851.3018507E-859.724174145
AGGTACT1206.002665E-1154.2908944
GCAGGGT701.7108094E-551.7086078
AGCGAGC450.00962703148.34814145
CAATTCG2550.048.2697539
GCAGGTA1402.728484E-1046.5916982
TTTAAAC2900.044.94343
Produced by FastQC (version 0.11.5)