FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_C09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_C09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATATTAAGTGTTTGCAGGATGCCCCATCGCACTTCTTCTTCTTTC11.1764705882352942No Hit
ATTCACTTGTTTTCATTCACTGGAGAGGAGATGGCCACCAAAGCGGCCTA11.1764705882352942No Hit
CTTCTACTAGATCGCACAGCCTCACTGAGTCCTGGTAGGATGATGGGCAT11.1764705882352942No Hit
GTTCAAACACGCACGTAAATAATACAGTGGACAATAATTCAACGCCACCC11.1764705882352942No Hit
GCATTTGATTGCATCATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGT11.1764705882352942No Hit
GGGGGAAGCAATCCACAACACGAGATCAAAATGCTTCAAAAAATAAGAAG11.1764705882352942No Hit
CTTCTAATAGATCGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT11.1764705882352942No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTAACGCAATGGAT11.1764705882352942No Hit
TCGCCAGGAACATTCGAGGATTTTGATTTTCATTCCACTTAGTGTTGTCC11.1764705882352942No Hit
GAATTGCCTCTTACCAGTATACTCAACCCTCATCCTCTCACAGTCACAGT11.1764705882352942No Hit
CCATCATTGCCCTCTGGGCAGCTGTTTGATATTTTCCTTTGACGATATAG11.1764705882352942No Hit
GGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTC11.1764705882352942No Hit
CCAGAGGTGATACCATCACTCGGTCTGATCCAGCATCGTTTGTTTTGCTC11.1764705882352942No Hit
TTCCCTAGTCTTGCCATTTTGTTTGAGAACATTATGGGTGCCATGCTCAG11.1764705882352942No Hit
CTTCGGGAGACAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAAT11.1764705882352942No Hit
CACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAA11.1764705882352942No Hit
AAATAGAGATAACAACCCACTTTCAAAGAAAAGGGAGAGTAAGAGCAAAC11.1764705882352942No Hit
GACAACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGC11.1764705882352942No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA11.1764705882352942No Hit
GCTTATAATAGCACTAACAATTAGATGTTGTTGTAAAAAAGCATCAATAA11.1764705882352942No Hit
GCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGAGAGTTGATACA11.1764705882352942No Hit
CCCACATCATTGCTGACGAATAAGTTATTGTCAACTTCTCAGTTCCATGC11.1764705882352942No Hit
GATTTTATTTTGTTGCCTTTCTCTAGGTAATATATGTGGAATTCCATCCT11.1764705882352942No Hit
GCCGTATTGCATTCAGTTTGGCCCCAGCTTGCTAGAGATCTGGGCTCTGG11.1764705882352942No Hit
GAGTAACACGGAGGGAAGTCCACATATATTACCTAGAGAAAGCCAACAAA11.1764705882352942No Hit
GCCCTCAAACCAGCCGTTAGGCTCGAATCCATATACATAGGCTGTAAAGT11.1764705882352942No Hit
GTTTACCATGTTTGTATCTTGTTAACCGTTTAGCTTATCATGGTCCAAGG11.1764705882352942No Hit
GAACAGAGTATGATGCAATTTTCCTCATTGACTGTGAATGTGAGAGGATC11.1764705882352942No Hit
GTTCTTCTCTTGCATTACTGATGTGTACTTATAGATTATGCCCATATGGC11.1764705882352942No Hit
ATCTAGAGGCCTATTTGGGGCCATTGCCGGTTTCATTGAAGGGGGGTGGA11.1764705882352942No Hit
CCCAGATATAGCAGTGTTGACGGAAAGTGGATGAGAGAACTCATCCTGTA11.1764705882352942No Hit
GCCCAGAGGGCAATGATGGATCAAGTAAGAGAAAGTCGAACCCCAGGACA11.1764705882352942No Hit
CGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAAAGTGTCTTT11.1764705882352942No Hit
GGTGGGTCTATTGGAAAATGAAAGAACTTTGGACTACCACTATTCAAATG11.1764705882352942No Hit
AACTAACTCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAA11.1764705882352942No Hit
TCGCAACACCTGGGATGCAGATTAGAGGTTTCGTATACTTTGTTGAAACT11.1764705882352942No Hit
CATCAACCTTTTCGCAAATCCTCATAGAAAAAGTATCAACAAAGAAAAAC11.1764705882352942No Hit
CACTAGAACAAAAGCACATTCTCTGGTCACTTATCCAATAATGTGGTATA11.1764705882352942No Hit
GACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATACTCATCATCCA11.1764705882352942No Hit
GTAAACAATGCTGTGGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGA11.1764705882352942No Hit
GTTGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACGT11.1764705882352942No Hit
ACATGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGA11.1764705882352942No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA11.1764705882352942No Hit
ACCTACTTATGAGTATGTAAAAAATTAATTTGGGGTAATCATCTGAGGAA11.1764705882352942No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC11.1764705882352942No Hit
CCTCTTACCAGTATCCTAAACCCTGATCCTCTCACATTCACAGTCAATGA11.1764705882352942No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA11.1764705882352942No Hit
CCTTGAAACAATGGAAGTTGTTCAACAAACAAGGGTAGATAAACTAACTC11.1764705882352942No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC11.1764705882352942No Hit
GGCCAGTTTTTGTATCAACTCTCCTCCTTATTTTAACTTGATTTCTGAAG11.1764705882352942No Hit
GTGGAGACGCATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTG11.1764705882352942No Hit
CACATTCAAATCTTTTCCTTCACTGGAGAGGACATGGCCACCCCAGCCGA11.1764705882352942No Hit
GTTTCATGCATTCACTATGAGAGCAAAATCGTCTGATGATTGGAGCACAT11.1764705882352942No Hit
GAGAATGGGAGACCTTCACTACCTCCAGAGCAGAAATGAAAAGTGGCGAG11.1764705882352942No Hit
CCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGT11.1764705882352942No Hit
GGAGTATTCATCTACTCCGATTTTGCTGACTCTTATCCGTCTCAGCGACA11.1764705882352942No Hit
TAAATACGATGACCTAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGGGCT11.1764705882352942No Hit
TTTCTGAACCACTCGGGTTGATTTCTGTTGATATATGTAATCATCGCCAG11.1764705882352942No Hit
ATTGCCGGGCCGCGGGGCCGCGGGCGGGGGGGGGGGGGGTTGGGGGGGGG11.1764705882352942No Hit
CCAGTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGA11.1764705882352942No Hit
CATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAGAT11.1764705882352942No Hit
CATAATTAATGAAAACAGCAAGCTGCATGCAACAAATACTGCAAATGCGA11.1764705882352942No Hit
CTATACCACCATGAAACGGCCAGACGAGATACCATCAATCGGTCTGATCC11.1764705882352942No Hit
CTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGGAG11.1764705882352942No Hit
TAGGAGACATGATCTTGAGGACTGCGATAGGCCAAGTGTCGCTGCCCATG11.1764705882352942No Hit
CAGTTGAGAAAAGCAAGATTTCTCTATTAATGCCCGGAAGGATTAGTGGA11.1764705882352942No Hit
TCAATGAACAAAGAGGAAATATAGATAACAACCCACTTTCAAAGAAAAAG11.1764705882352942No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC11.1764705882352942No Hit
CCTCGTGACTCGGGTCTTCAATACTTAATTTCAGAGCATCCATCAGCAGG11.1764705882352942No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTGCCTAGCGGTTGATA11.1764705882352942No Hit
GTGGTAGTGAGTATTGCCCGATTTTTAAGGGTTAGAGATCAAAGAGGGAA11.1764705882352942No Hit
GGCAGGAAGTCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACA11.1764705882352942No Hit
AGATATTGTCAACTTCTCAGTTCCTTGCGTTTCACTGACTTCTTCGGGAG11.1764705882352942No Hit
AACTCAAGGTCGCCAGACTTATGATTGGACATTAAACAGAAATCAACCGG11.1764705882352942No Hit
TGCTAACGGTCAACCTCCCAACACTGAAAATAAGAGTACATGAATTGTAT11.1764705882352942No Hit
AGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATT11.1764705882352942No Hit
GTGTTACATATACTGTTCACCACTGTCCAGGCCATGATTCGGTCTCTTCC11.1764705882352942No Hit
TTCCAGTCCTCCGATGAGGCCCGCAACTGCATTTTTGACATCCTCATAAG11.1764705882352942No Hit
TCTTCATTCTTTTACTCTCGAACATGTACCCTTTCCCTAGTCTTGCCATT11.1764705882352942No Hit
CTTTTTTTATCGCTATGTATTTGTGGCTAATTTTAGCATGGAGCTACCCA11.1764705882352942No Hit
GAGTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCGTTAGCG11.1764705882352942No Hit
GGGGATTACAAAGTCTTCCCCGATAATAATCATACATTAGCTCCCATTTT11.1764705882352942No Hit
GCAGATGGGCACGCAAAGAAGAAAACTCAAGAAAAACCAAAGACTGAAAA11.1764705882352942No Hit
CTTTATCACTGTTACTCCAATACTCATGTCAGCTGATTCATTTACTCCAG11.1764705882352942No Hit
CTCTACCCCTGTTGTGTTACATATACTGTTCACCACTGTCCAGGCCATGA11.1764705882352942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATTC50.0145.07
TTCATGC50.0145.03
CATCCCC50.0145.0145
TTTCATG50.0145.02
GTTTCAT50.0145.01
CATTCAC50.0145.09
TCATGCA50.0145.04
ATGCATT50.0145.06
GCATTCA50.0145.08
CATGCAT50.0145.05
Produced by FastQC (version 0.11.5)