FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_C08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_C08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences865594
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2900.050.033811
CTATTCG803.7563794E-545.2671559
GCGAAAG3900.037.1766552
TCGTATA800.002037224636.247242
CGAAAGC4000.036.2262843
GTATCGA850.002732707734.1407171
CGTATAC1000.00609346228.9810283
TTACGCA2502.4701876E-928.9726524
CCTCGCG1701.0292373E-425.59517145
TACGCAG3052.0543666E-823.7480775
GAGCGAA2202.2167334E-523.083781
TTACGGG2202.2466744E-523.0437666
TGTTACG3203.3869583E-822.6545242
GTATGGC2252.671592E-522.5329867
ATTACGG2353.7355192E-521.575385
TATAATA5051.8189894E-1221.533012
GTATTAC7450.021.4238721
GTTACGC3406.4736014E-821.309583
GCATTAG4151.0822987E-920.9780311
CATTACG2104.3410307E-420.6947544
Produced by FastQC (version 0.11.5)