FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_C07_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_C07_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA80.33112582781456956No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG80.33112582781456956No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA70.2897350993377483No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA70.2897350993377483No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA60.24834437086092717No Hit
CCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT60.24834437086092717No Hit
CTTCTGGTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGA60.24834437086092717No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG60.24834437086092717No Hit
ACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACAT50.20695364238410596No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA50.20695364238410596No Hit
GCCTAACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAGGATGTA50.20695364238410596No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG50.20695364238410596No Hit
CTAATAGCCCTACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCA50.20695364238410596No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT50.20695364238410596No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC50.20695364238410596No Hit
GTTCATTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA50.20695364238410596No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT50.20695364238410596No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAG40.16556291390728478No Hit
GTATACGAAACCTCTAATCTGCATCCCAGGTGTTGCGATAGCCCTTCTTT40.16556291390728478No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA40.16556291390728478No Hit
ACTGAACCCCATGCACCAACTCTTGAGGCATTTCCAAAAAGATGCAAAAG40.16556291390728478No Hit
ATGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGC40.16556291390728478No Hit
GGTATGAAGAATTCACAATGGTTGGGAGAAGAGCAACAGCTATTCTCAGA40.16556291390728478No Hit
GTGTTGGCCAATGCAGTTGCTGCCGGTTGATTTCTGTTTAATGTCCAATC40.16556291390728478No Hit
AGTCAACACTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTC40.16556291390728478No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.16556291390728478No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT40.16556291390728478No Hit
GCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAAATGAGAAATC40.16556291390728478No Hit
GTTTCAACCTTTCGACCTTTTCGAAATAAGTTTTATATACCTTAGGGTAA40.16556291390728478No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG40.16556291390728478No Hit
GTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGTCAGCTG40.16556291390728478No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA40.16556291390728478No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC40.16556291390728478No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT40.16556291390728478No Hit
ACCTTTGATTGGGTTTGATCCCACAGCTTCTTTAACTCAAATGATCTTCT40.16556291390728478No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG40.16556291390728478No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTT40.16556291390728478No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG40.16556291390728478No Hit
CCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTG40.16556291390728478No Hit
GCATCACTGAGTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTAC40.16556291390728478No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT40.16556291390728478No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT40.16556291390728478No Hit
ATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAG40.16556291390728478No Hit
GCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCA40.16556291390728478No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG30.12417218543046359No Hit
TGGTACCTTCGGCCCTGTCCACTTCAGAAATCAAGTTAAAATAAGGAGGA30.12417218543046359No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA30.12417218543046359No Hit
GTCATAAAGAGATTGATTCTGTAAACAATGCTGTGGTAATGCCAGCCCAT30.12417218543046359No Hit
GCACACAGATTGGAGGAGTAAGGATGGTGGACATCCTTAGACAGAATCCA30.12417218543046359No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG30.12417218543046359No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC30.12417218543046359No Hit
ATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGCAGAAAT30.12417218543046359No Hit
CTTCAATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTT30.12417218543046359No Hit
CCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGAAG30.12417218543046359No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT30.12417218543046359No Hit
CCCCAGCTCAACCCGATTGATGGACCACTACCTGAGGATAATGAACCAAG30.12417218543046359No Hit
ATTCTCTTTCTAGCATGTACGCCACCATCAAGGGAGCAATTTTACAATCC30.12417218543046359No Hit
CCCTTGATGGTGGCGTACATGCTAGAAAGAGAATTGGTCCGTAAAACAAG30.12417218543046359No Hit
CTCCTGGAGTGTACATCTGCTCCCAGCACGTCCCTTGGGTTAAGTGCAAC30.12417218543046359No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG30.12417218543046359No Hit
CTCTTGGAAATGTGCCACAGCACACAGATTGGAGGAGTAAGGATGGTGGA30.12417218543046359No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA30.12417218543046359No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT30.12417218543046359No Hit
GGGTACATGTTCGAGAGTAAAAGAATGAAGATTCGAACACAAATACCAGC30.12417218543046359No Hit
CTCCCGCTTACTATCAACTGGATCAATCTCCTGGTTGCCTTTCTGAGAAT30.12417218543046359No Hit
CTCTTATCCCTCTCAGCGACATCTCCGTGCTTGGGGTCATGTCGGGCAGT30.12417218543046359No Hit
GTTCAACATGCTAAGTACGGTCTTGGGAGTCTCGATACTGAATCTTGGAC30.12417218543046359No Hit
GAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACCGATTTTTAAG30.12417218543046359No Hit
GGCATTACCACAGCATTGTTTACAGAATCAATCTCTTTATGACTGACAAA30.12417218543046359No Hit
CTATTTCCTCTTTGTTCATTGATTCCATTACATCCTTTAAGAAATCTATT30.12417218543046359No Hit
GACATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTT30.12417218543046359No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA30.12417218543046359No Hit
CCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAAATTGCATCC30.12417218543046359No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCAT30.12417218543046359No Hit
GCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGAT30.12417218543046359No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG30.12417218543046359No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG30.12417218543046359No Hit
GGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATT30.12417218543046359No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG30.12417218543046359No Hit
GTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTC30.12417218543046359No Hit
CACAAATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCTGTGGGATCAA30.12417218543046359No Hit
GGGTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACC30.12417218543046359No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA30.12417218543046359No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG30.12417218543046359No Hit
TATATTACCTAGAGAAAGCCAACAAAATAAAATCTGAGAAGACACACATT30.12417218543046359No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG30.12417218543046359No Hit
GTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGG30.12417218543046359No Hit
GAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGGACATACT30.12417218543046359No Hit
TCCCAAGACCGTACTTAGCATGTTGAACATGCCCATCATCATCCCAGGAC30.12417218543046359No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATCTAAAGACT30.12417218543046359No Hit
ACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAAAAACAAAGACT30.12417218543046359No Hit
GAGACAACATGACCAAGAAGATGGTCACGCAAAGAACAATAGGGAAGAAA30.12417218543046359No Hit
GTTCTATCCAGCTTGAATCAGTCAATTCACATGCCTTATTGAATTCATTT30.12417218543046359No Hit
GGCCAAACTGGCAAATGTTGTGAGAAAGATGATGACTAATTCACAAGACA30.12417218543046359No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT30.12417218543046359No Hit
TCGGAATACTGCCCGACATGACCCCAAGCACGGAGATGTCGCTGAGAGGG30.12417218543046359No Hit
GAACAATAGGGAAGAAAAAACAAAGACTGAATAAGAGAGGCTATCTAATA30.12417218543046359No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGT100.0066591203145.0145
Produced by FastQC (version 0.11.5)