FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_C06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_C06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062331
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATAAATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC28430.2676190377575351No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAATGCATGTGTT25720.2421090978235597No Hit
GATCTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC24950.23486088610800213No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCTGCTGT22400.21085706808894777No Hit
ATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTATGA22270.20963334403307443No Hit
CTTAATATGAGTGCAGACCGTGCCAGAAAGGTGAGATCTTCGATCTCAGC21790.2051149782883113No Hit
GCATAGATCTCATAGGCAAATGGTGACAACAACCAATCCATTAATAAGAC18150.17085070472385727No Hit
CTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCATTCT17910.16859152185147566No Hit
CGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCGATCAAGAATCCA17900.1684973892317931No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG17760.1671795325562372No Hit
GATTTATGCTGGGAGTCAGCAATCTGTTCACAGGTTGCGCATATTAGGCC17410.16388489086734737No Hit
CTCATAAGCAATCCTTGTTTTTCTTCCATTCTCACCCCTCCAGAAGTTTC16870.1588017294044888No Hit
ATATACAACAGGATGGGGGCTGTGACCACCGAATCGGCATTTGGCCTAAT16860.15870759678480623No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA16800.15814280106671083No Hit
CTCATAGACTCTGGTACTCCTTCCGTAGAAGGCCCTCTTTTCAGACCGCG16270.15315377222353485No Hit
TGTCAAAGGAGGGCACGATCGGGTTCGCTGCCCTTTCGTCCGAGAGCTCG15880.14948260005591477No Hit
AGCAGGTACTGATTCGAAATGGAAGATTTTGTGCGACAATGCTTCAATCC15740.14816474338035884No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA15370.14468183645210392No Hit
ATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGAAC15330.14430530597337365No Hit
CATTATGATCTGGCATTCCAATTTGAATGATACAACTTACCAGAGGACAA15320.14421117335369107No Hit
CCCATATACAAGAGAGTAGATGGAAAGTGGATGAGGGAACTTGTCCTTTA15150.14261091881908747No Hit
CATTAATACTGCTTTGCTTAATGCATCCTGTGCAGCAATGGATGATTTCC14800.13931627713019765No Hit
CATATTAAGAGGGTCAGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT14780.13912801189083251No Hit
TAATAGTAGAGCCTGAAGACCCAAACGCACTTTTGAAGCACAGATTTGAA14590.13733949211686378No Hit
GTTGTAAAATGAAATGGGGAATGGAGATGAGGCGTTGCCTCCTCCAATCA14340.13498617662479961No Hit
CTTTTCAGACCGCGTTTAAGGAGACGATAAATGCATTTGAAAAAAAGGCG13900.1308443413587667No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAGCTATCTCAA13760.1295264846832108No Hit
AATATGGACAGAGCCGTTAAACTGTATAGAAAGCTTAAGAGGGAGATAAC13270.12491398631876505No Hit
GTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAA12950.12190174248892295No Hit
GTATGGAGAGGATCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA12440.11710097888511208No Hit
GTTTTTTACTCTAGCTCTATGCTGACAAAATGACCATCGTCAGCATCCAC12410.11681858102606438No Hit
GTCGTACTCCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCA12300.11578312220955615No Hit
GCTTATGAGAGAATGTGCAACATTCTCAAAGGGAAATTTCAAACAGCTGC12250.11531245911114332No Hit
GTATAGAAAGCTTAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATAG12080.1137122045765397No Hit
GATTTGAAATAATAGAGGGAAGAGATCGCACAATGGCATGGACAGTTGTA11970.11267674576003148No Hit
GAACAATAAGGAGCAATTTTCTTGATTCAGCTGAAAATCCTTCTAATTGT11820.11126475646479297No Hit
CACTTGGAAGTATGCTTCAGATTTTCATTTCATCAATGAGCAAGGCGAGT11770.11079409336638016No Hit
GTTCCAGATTGTCCCTAAGAGCCTGAACAATAAGGAGCAATTTTCTTGAT11640.10957036931050682No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCCAGATTGTCCCT11530.10853491049399858No Hit
CTATGGTATCAAGTACTCTTGAACTGAGAAGCAGGTACTGGGCCATAAGG11520.10844077787431601No Hit
ATCTCAATGCATGTGTTAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAA11200.10542853404447389No Hit
ATACCATAGTATCCATGTTTTCATTTGAAGCAATTTGTACTCCTCTAGTG11050.10401654474923541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGCG202.1279866E-6145.03668145
AGCGAAA950.0122.1995241
GGGTAGA1050.096.7412951
GAGAGGT259.377479E-487.02201145
TCGGTCC259.377479E-487.02201145
GCTCACG451.2310848E-680.575935145
TGGTAAC300.001929857472.555981
GCCGACC406.655028E-572.51834145
GTAGAAA5900.067.636921
TAGATAT3800.059.0816963
GGCAGCC502.0077883E-458.01467145
CTACTAA400.00608109854.306826
ATGTGCG553.2150734E-452.740612145
GTACTGA4050.051.8484886
TTAGTAG701.6883268E-551.8256951
TACTGAT4100.051.2113657
TTCGTAA901.3213466E-648.345562145
CGTGTAA450.00962966848.345562145
CTGTCTA604.939054E-448.34556145
ACACATA604.939054E-448.34556145
Produced by FastQC (version 0.11.5)