FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_C05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_C05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGGGGTCCTCATCGGAGGACTTGAATGGAATGGTAACACGGTTCGAGT15.555555555555555No Hit
CCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTC15.555555555555555No Hit
AGCTCCAGGATTGTAAAATTGCTCCCTGGATGGTGGCGTACATGCTAGAA15.555555555555555No Hit
GTCAATTCACATGCCTTATTGAATTCATTTTGGACACCGCTTTCTAGCTA15.555555555555555No Hit
GCGAACTTCAGTGTGATTTTTGACCGGCTGGAAACTCTAATATTACTAAG15.555555555555555No Hit
CTTGAACCGGATATTTTGTATTACTGATATTCTTTTTTTCAAATGTATTT15.555555555555555No Hit
GAACAATTCAACAGACACTGTAGACACAGTACTAGAAAAGAATGTAACAG15.555555555555555No Hit
GAGTAAGTATGGTGTACATCCTTAGACAGAATACAACTGAGGAACAATCC15.555555555555555No Hit
GTATGCTAGAGTCCCGTTTTGGTTTCATTTCACACACTAAGGGCCCATGC15.555555555555555No Hit
GTGTCAGCAGACAAATTAGCATCTCGCTTGGAAATGTGCACCAGCACACA15.555555555555555No Hit
GGCTAATGTGCTAATTGGGCAAGGGGAAGTATTGTTGGTAATGAAACGAA15.555555555555555No Hit
TTTCTAGTTCAATCAGTAGTTGTAACGCTTGCTTAAATGTTATTTGTTCG15.555555555555555No Hit
GCCCCCTACGGGGAGCCCAGACTGTTCAAGATTTTCGCAAATGCTCCTAG15.555555555555555No Hit
GTGGGGGGGTGTGGGGGGGCGGGGATGCTGGCGCGGGGCTCGGGGGCGGG15.555555555555555No Hit
TACAAACCCTGGCCATGCAGCTCTCAGTGCCAAGGAGGCACAGGATGTGA15.555555555555555No Hit
AGACATGACCAAGGAATTCTTTGAAAACAAATCGGAAACATGGCCAATCG15.555555555555555No Hit
CCTTAATGCAGCCGTTCGGCTCGAATCCATCTACATAGGCTCTAAAGTTT15.555555555555555No Hit
GTATTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAATCACATCC15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)