FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_C01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_C01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGATGGCTATTCAAATGGTCTTCAAAGACTACAGATACACAGATAG13.571428571428571No Hit
GATTTGTATGATTACAAAGAGAACCGGTTCATTGAAATTGGAGTAACACG13.571428571428571No Hit
ACATTAAAGAGTATAAGAATCGAACTCAAAAATATACTAATATCACAACC13.571428571428571No Hit
CCACATAACTATGTCCTGGAAGAGAATGTAATCTTGAAATTTCTCAAACT13.571428571428571No Hit
GGGGAGAAGTATTATTTGTTGTCCAGGGTCAAATGTCCCAACCAAATAAC13.571428571428571No Hit
GGGGGATATAAAACCTTCGAATGCTATATAGCGAGAGACCTCAGTTCTGA13.571428571428571No Hit
GAATAAGAGAGCCAATCTAATAAGAGCACTGACATTAAATACGATGACCA13.571428571428571No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA13.571428571428571No Hit
AAACTGCAAAAACATGACACAAAGTTGACCACAACAGCACTCATCAGACA13.571428571428571No Hit
CATGATTGGTGCAAGGAGCAGGATGTTTCTGCACCCAGCGGTGTGAGTTC13.571428571428571No Hit
CTCCACTCACCTGGGTGACTCGCCGATTGGCCATGTTTCCGATTTGTTTT13.571428571428571No Hit
GTAGAAACAAGGCAATTATTCATGAAAGACAACTTAAATTCAATATTATT13.571428571428571No Hit
ATGAATAACATTCTTGGTACTTGTCAGCAAACTCATAAAACACATACCCT13.571428571428571No Hit
TCCCAGGGTCCACTAAGAGTGTGGACGGATAAAGAAAGAAGAGTTCTCTC13.571428571428571No Hit
GGGGAGGAGAGAGGAGAGGTAGTGAAGAAGAAGGTGTGTGAGTGGTTGTG13.571428571428571No Hit
GCAAAGGGGAGAAGTTTTATTATTTGGACAGTGTCAAATGTCCCACGCAC13.571428571428571No Hit
CTAAGACCCCTGTGGACAATATGGCCATAATCAAAAAGTACACAGCAGGA13.571428571428571No Hit
TCCGACGATTTTGATCTCATAGTGAATGCCCCAAACAATGATGGAATACA13.571428571428571No Hit
GACTTAAGGAATCTGAAGATTCCGTATATTGTACTCTAATACCTCTTTGT13.571428571428571No Hit
CATCAGTATAATATCTAAAATGATACCTTAATTTTATAATGAACACATAC13.571428571428571No Hit
AAACAACGTCGTTGTTAAACAATTCGCCACTAATTGATCGACATCCGAAT13.571428571428571No Hit
GGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGGGTCTGGAGTAAATG13.571428571428571No Hit
ATGGAATCCCACAAAGTGACATCGCCCTGCAATGGTGAATGTGGTGGATA13.571428571428571No Hit
GTTTTAATTCTTTCCATTCACTGTTCCTCTAGGGAGTAAACAGCATGCTT13.571428571428571No Hit
CTATTGAATACTGACTTAGCTAATAACGTCCTGCATACTTTCCCAATGGA13.571428571428571No Hit
CCCAATCAAAGGTATGGATATTAGGATAAGATGGAGGACCAAACTTATAC13.571428571428571No Hit
GCATCAAATATCGCCCCAAGAACGAATCTACCAGGTTACAGGTTTACCCT13.571428571428571No Hit
GATCTCAGTGCCAAGGAGGCACAGGATGTGATTACTGTCCAAATAATAAA13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)