FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_B09_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_B09_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC22.3255813953488373No Hit
CAGTAGGATTCTGTCTAAGGATGTCCAAAATCCGAACTCGTCCAATATCT11.1627906976744187No Hit
ATCACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGGGCAGGGTTTGT11.1627906976744187No Hit
TCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTATTTTGTCTCCCG11.1627906976744187No Hit
TCAATGAACTGAGCAATCTTGCTAATGTGCTAATTGGGCAAGGGGACGTA11.1627906976744187No Hit
CGAATTGATGGCCATCAGAATTCTTTTGGTAGGTGTCGGGCTGTCGGGAG11.1627906976744187No Hit
ATCATGGATCAGAAAAAGTGATAATATCAAATATCCCAGTAACATTGTGT11.1627906976744187No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1627906976744187No Hit
ACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAA11.1627906976744187No Hit
ATTCCATGCTTTTGGCTGGACCATGGGCTGGCCATACCACAGCAGTGTTT11.1627906976744187No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG11.1627906976744187No Hit
GTAGAACCAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTT11.1627906976744187No Hit
ATCAGGCACTCCTCGATTGCTTCATATAGCCCCCCAAGATCGAAGGTTCC11.1627906976744187No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA11.1627906976744187No Hit
CAATCCGAATTATTTTGGTCGCTAACACGCTTTGAGTAAGTATGCTAGAG11.1627906976744187No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.1627906976744187No Hit
GCTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAA11.1627906976744187No Hit
GAACATCCCAGTGCTGGGAAGGACCCTAAGAAAACATGAGGACCCATAAA11.1627906976744187No Hit
GAATCGAACTGAGGAACAAGCCGTAGACATATGCAAGGCCGCAATAGGGT11.1627906976744187No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC11.1627906976744187No Hit
TGGGATGGGAAGGATCGGAATCCTGCCCGACCTGACCCCTAGCAGGGAGA11.1627906976744187No Hit
GTTCTCTTCCAGATTTTTTTATAACCACTTTGACAAAGGAAATCATAAGG11.1627906976744187No Hit
CACAAATTCAGACGAGAAGATCATTTGAGTTAAAGAAGCTGTGGGATCAA11.1627906976744187No Hit
ATGATACTGATGTCGTGAACTTTGTAAGTATGGAGTTCTCACTCACTGAC11.1627906976744187No Hit
GTACTCCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGAC11.1627906976744187No Hit
CCAGGAGATTGATCCAGTTGATAGTAAGCGGGAGAGACGAGCAGTCAATT11.1627906976744187No Hit
GTCTGGAGCCGCAGGTGGTGCGGTGACAGGGGTTGGGACAATCGGGAGGG11.1627906976744187No Hit
GGGCATGACTTTGAAAGGGAAGGGTACTCACTGGTCGGGATAGCCCCATT11.1627906976744187No Hit
GACAATGTGATGGGAATGATCGGAATACTGCCCGACATGACCCCAAGCAC11.1627906976744187No Hit
TAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTCGATC11.1627906976744187No Hit
TGTCGATACATCTCAATCTTAAGACAATGCACCTGCATACTATCCAAGAA11.1627906976744187No Hit
AGCTAAAGTTTCAACAAAGTATACGAACCCTCTAATCTGCATCCCAGGTG11.1627906976744187No Hit
CAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCA11.1627906976744187No Hit
GAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACAAAGAAGAA11.1627906976744187No Hit
CTATTAGAAGAGGCCTTATTTTCTCACTTTTCTTTTTTGTTGATTCATTG11.1627906976744187No Hit
GTAAAAATCAGGAAGGCAAGAGAATCAAACCGCACTCAGAATGAAGTGGA11.1627906976744187No Hit
AAATTATGCATTAATTGAAAAAGGAACGCCATAAGTATAATTCCCAATTT11.1627906976744187No Hit
CACTGATCTGGCCTGCGGATGCCTGTTGTTGATTGGTATTTCCTCCACTC11.1627906976744187No Hit
GTCTAAGGGCTTGCAGTAGCAAGTGGACATGACTATGAAAGGGAAGATTA11.1627906976744187No Hit
TAGCAAGATTGCTCAGTTCATTGATGCTTAGCAGACTAAACCCCAGATGT11.1627906976744187No Hit
AGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTG11.1627906976744187No Hit
ACTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGAAG11.1627906976744187No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG11.1627906976744187No Hit
ACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGAAAAG11.1627906976744187No Hit
GGGTATTTTTCCTCAACTGTCATACTCCTCTGCATTGTCTCCGAAGAAAT11.1627906976744187No Hit
TCAATGAACTGAGCAATATTGCAAAAGGAGAGAAGGCTAATCTGATAATT11.1627906976744187No Hit
AATATGCACACATTTGGAAGTTTGTTTAACTTTAGGGGTTTTCGGCTGAA11.1627906976744187No Hit
TTGCATCTGTACCTACTTCGAGCTACCTTTCTGACATGACCCTCGAGGAA11.1627906976744187No Hit
ATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGGCTCTT11.1627906976744187No Hit
ATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACGG11.1627906976744187No Hit
GAGCGAAAGCAGGCAAACCATTTGAATTGATGTCAATCCGACTCTACTTT11.1627906976744187No Hit
GAGTACCCTTCCCTTTAAAAGTCATGCCCACTTGCTACTGCAAGCCCATA11.1627906976744187No Hit
TCTTTCCATTGCGAATGCATATCTCGGTACCACTAGATTTCCAGTTGCTT11.1627906976744187No Hit
GATTGGAGGAGTAAGGATGGTTTTCATGGTTTGAAAGAATCCAACTGAGG11.1627906976744187No Hit
GAGAATAAGAGATATGGCCCAGCATTAAGCATCAATGAACTGAGCAATCT11.1627906976744187No Hit
GACAGAGCAGCCCATGGAGTTAATGCTGGCCTATCTCAAGGACCAATTTG11.1627906976744187No Hit
GACCATTGACCGAATTTTAAGGGGTAGATATCAAAGATCGATCGTAATAT11.1627906976744187No Hit
ATATGATGCAATCAAATGCAGCAAGACATTCTTTGGCTGGAAAGCGCCTA11.1627906976744187No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGAAGCACTTCT11.1627906976744187No Hit
ATTCTACACTAATTGATTGACATCATAAAAATTTTGGTCTCGCAATTGAT11.1627906976744187No Hit
CCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTAT11.1627906976744187No Hit
GATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTC11.1627906976744187No Hit
AAACAAGGTCGTTTTTAAAAAATAAGACAATAATTGAAGGCCATCCGAAT11.1627906976744187No Hit
GATTGTCTCCTAAAATACCACTGCATATGTATCCTATCTGATATTACAGA11.1627906976744187No Hit
CAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATAC11.1627906976744187No Hit
GTCTAATAGATTTCTAAAAGGATGTAATGGAATCAATGAACAAAGAGGAA11.1627906976744187No Hit
GTACATCTCTTCATCCTCAAGAATTACCCTTTGGCTTGTGTTGAGAATAG11.1627906976744187No Hit
TACTATATGTGAGAACCAATGGAAACTACAAGATCAAGATCAAATGGGCC11.1627906976744187No Hit
AACTTATACAATATACCGAATATTCACAATCAACAAGTCTGATTAAAATG11.1627906976744187No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG11.1627906976744187No Hit
CACCATTACACAGTAAACAGAACACACCAATACTCAGAAAAGGGAAAGTT11.1627906976744187No Hit
GCTTAAGGCTGGGCCATATCTCATGTCTTCTGTGGCCAAAATGAGATATC11.1627906976744187No Hit
ATATAAACATCAGTGATGAATCGGAGCTGGTTAGTATCGTTGTTTGAATC11.1627906976744187No Hit
AAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAAGGCACCCAGAAG11.1627906976744187No Hit
GGCATCAATCCGGGCCCTAGACACCATGGCCTCCACCATGAGAGAAATTC11.1627906976744187No Hit
TGCCACTTGTGAACAGATTGCTGCTTCACAGCATCGGTCTAACAGACAGA11.1627906976744187No Hit
ATATCAAAGACAATCCAACTTGATAATCCAAGCCAATACACACAAGCACG11.1627906976744187No Hit
ATCCTCAAAGGAAAATTTCAAACAGCTGCCCAGAGGTCATTGATGGATCA11.1627906976744187No Hit
ATAAGGTAGACTGTTTCGTTTGGCATATCCGCAAGCCATTTGCAGACAAT11.1627906976744187No Hit
TACCAACACTACGTCCCCTTGCCCAATTAGCACATTAGCCTTCTCTCCTT11.1627906976744187No Hit
GAGTACCCTTCCCTTTCAAAGTCATGCCCACTTGCTACTGCAAGCCCATA11.1627906976744187No Hit
GGGCCCTCTGGAATCATGTCCAATATTCTCTTGTCTGCTGTAATTGGGTA11.1627906976744187No Hit
GCAGATTGAGAGCATGATTGAGGCCGAGTCTTCTGTCAAAGAGAAAGACA11.1627906976744187No Hit
CCACAATATCAGGTGCAAGATCCCAATGATATTTGCTGCAATGACGAGAG11.1627906976744187No Hit
TCACGAGACTGGTTCATGCTCATGCCTAGGCAAAAGATAATAGGCCCTCT11.1627906976744187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTTGG50.0145.09
CTTCAGG50.0145.0145
CCATGCT50.0145.04
TGCTTTT50.0145.07
TTCCATG50.0145.02
ATGCTTT50.0145.06
TCCATGC50.0145.03
ATTCCAT50.0145.01
GCTTTTG50.0145.08
CATGCTT50.0145.05
Produced by FastQC (version 0.11.5)