FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_B08-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_B08-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1107142
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA16910.1527356021178855No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG15410.13918720453202932No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT14630.1321420377873841No Hit
GTATATATGTATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAA11990.10829685803627719No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2600.039.057621
TATACCG1251.2703707E-534.779323
TATACAG11900.030.4317045
GTATATA25700.027.6594811
ATATATG12950.026.8566153
ATATACT12700.025.0929784
CAGCTCG11050.024.907189
GTTATAC2701.6414015E-724.1785281
TATATAT16550.024.0902232
TATATGT14200.023.4624164
CGAAAGC4700.023.1245483
GTACACC4403.6379788E-1223.0544743
GTATTAA11250.022.5666241
TATACTG6100.022.5593725
ATGTATG14000.022.2445747
GTATAGG5001.8189894E-1221.7606751
GTATGTA13700.021.1467049
GTATACG3106.215814E-721.0587181
TATATAC28300.020.738553
GCGAAAG5301.8189894E-1220.5289361
Produced by FastQC (version 0.11.5)