FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_B05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_B05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC14.761904761904762No Hit
CTACAATTATTGATTCACCCCGTTCGTCGATGAAATGGAAATCCGAATAC14.761904761904762No Hit
ATTTACACATGCACATTCAGACTCTTGTGTTCTCAATATATTGTTTCTCC14.761904761904762No Hit
GGGTGGCGTGCCGGTGGGGGGCCTGGGAGGTTTTGAGAGTAGCGGGTGGC14.761904761904762No Hit
GGCTGCGTGCTCTCCTCCACTGTCCGGATAATCTTCGAGATCTGTTTANN14.761904761904762No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGGTTTCGGTTGGTTACC14.761904761904762No Hit
ATTTAAAGATGAGTCTTCTACCCGAGGTCGAAACGTACGTTCTTTCTATC14.761904761904762No Hit
GGTATTATCATTTAATCTCCACCATTCAAATCTTGCACTACAAATTGTAA14.761904761904762No Hit
ATCATGGCCTGGACAGTGGTGAACAGTATATGTAACACAACAGGGGTAGA14.761904761904762No Hit
TGAGATTGAGCAAGTTGATAGTACGCGGGAGAGACGAGCAGTCAATTGCT14.761904761904762No Hit
GAACCTTGACCGCTGATGGCAAAGAGGCCCATCAGGCAATCTGAGGGGGC14.761904761904762No Hit
GAGTCACAGCTGGCAATAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTG14.761904761904762No Hit
GTGTTGACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGA14.761904761904762No Hit
GGTCAATACGCAAGACCCCTCGCTCCCTGCTGGAGGCTTCCTCTACTCCC14.761904761904762No Hit
GCTGACATGAGTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAA14.761904761904762No Hit
GGCAATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGC14.761904761904762No Hit
CCAGGAGGAGAAGTGAGAAATGATGATGTTGACCAAAGTTTGATTATCGC14.761904761904762No Hit
ATATAGGACTTCTTTTTGATGATGTTTATTAAAACTAACTTCCAGGTCAT14.761904761904762No Hit
ATTCGACCCTAATTGATGGCCCTCCGGTTTCTTTTGGTCGCTGTCTGGCT14.761904761904762No Hit
GAATAGAACGATTCCTATTGGGAATCAAGTAATGTGTAGTTGAAACGGCA14.761904761904762No Hit
ATCTAATAAGAGCACTGACATTAAATACGATGACCAAAGATGCAGAGAGA14.761904761904762No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)