FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_B01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_B01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATGAAATTGCAAATGAAAAGAAGATAACAAGGACAACGAACATGCAGA12.1739130434782608No Hit
AGCAAGCTGGGTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGATT12.1739130434782608No Hit
GTGCTTATCAGCGACATCTAAGACATAAGCGTATAAGCGTCCAGTGGTTG12.1739130434782608No Hit
GTTCAAGCAAATTCGGGATGTGCTTGCGAAATTTGAAACTGTCCAAATAA12.1739130434782608No Hit
AACCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGACCTGCAA12.1739130434782608No Hit
GCACAGGATGCATTGAGCAAGGCCGTATTTATGTACACTCCCTTCATTAT12.1739130434782608No Hit
CATGAACCCTTTCCCTAGTCTTGCCATTTTGTTTGAGAACATTTTAGGTG12.1739130434782608No Hit
ACTATATGAACTGATAGGGAAAAATTTCTCTAATAGATTTCAGCACTTCT12.1739130434782608No Hit
ATATGGCCATAATCAAAAAGTAGACATGAAGAATGCAAGACAAGAAACCC12.1739130434782608No Hit
GCGAATGCATATCTCGGTACCACTAGATTTCCAGTTGCTTCGAATGTTAT12.1739130434782608No Hit
TAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTG12.1739130434782608No Hit
GAATTTGTGTGTCTCCCCTATGGCACCTATATGTGTATCTGTAGTCTTTG12.1739130434782608No Hit
CACTGTTCCAGCAAATGCGGGATGTGCTTGGGACATTTGACACTGTCCAA12.1739130434782608No Hit
NTCTGGTCTCCCAGTAGGGGGCAATGAAAAGAAGGCCACACGGGAAAAGG12.1739130434782608No Hit
ATAGTAGAAAGGCTCTTCACGACTTCTCAAGTAAAATATTCCAGACCCAA12.1739130434782608No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG12.1739130434782608No Hit
AGTCGTTGGTCATGTCTTTCTCTTTGACAGAAGACTCGGACTCAATCATG12.1739130434782608No Hit
ATTTTAAGCGTAAAATATCAAATAGAGACCTTACTATATTATCACGATGA12.1739130434782608No Hit
CAGCAACTCATACATTTGAAGCCACTCTTAACAAACAGTATTTAAAGCAA12.1739130434782608No Hit
ATTCAAATCTACACTTATAAAACCATAAACACGAACTGGTAAAATGAAAA12.1739130434782608No Hit
CTTAAAAAGTAAACGAGTCAATTTATAAAAAAGTCACAATAAAAAAAATC12.1739130434782608No Hit
CAATTGTTCTTTGCATGACCATCTTCTTGGTCATGTTGTCTCTTACTCTC12.1739130434782608No Hit
TCTTGTATTCCCTCATGGTTTGGTGCATTCACGATGAGAGCAAAATCGTC12.1739130434782608No Hit
GCCACAGAGATTTCTTTAACAAGCAATGTTGACAACACGAAGTGTAAGGA12.1739130434782608No Hit
ATGTTATACAACAAAATGGAATATGAACCATTTCAGTCTCTTGTCCCTAA12.1739130434782608No Hit
TTTATAATCTGTACAATCCGGATTTTATTACCTCATTGTTTTGTTCATTC12.1739130434782608No Hit
CAATACAGCCATGGAACAGGCACCGGATACACCATGGACACAGTAAACAG12.1739130434782608No Hit
ACAATATAGACAGTATATGTGATATTTTAGGATTTAAGAAATCCAGAAAG12.1739130434782608No Hit
GGAAAAAGCACAAAATTGAGCCTGGCCACAGGATTGAGGAATATCCCGTC12.1739130434782608No Hit
CTACAGATACACATATAGGTGCCATAGGGGAGACACACAAATTCAGACGA12.1739130434782608No Hit
ATGGGAGAGTCCCCCCGGGGAGTGGAGGAAGGCTCTATTGGGAAAGTGTG12.1739130434782608No Hit
ACGAAAAGCCAAGGAAAGAATATGGTGTTGATCCCAAATGATATCCTAAC12.1739130434782608No Hit
CATTAAAAAAGACCCCCAACAACGAAGATACCAGGTTCCAACTTATACCT12.1739130434782608No Hit
GAATCAAAACTAGGCTTTTAACTATAAGACAAGAAATGGCCAGTAGGAGT12.1739130434782608No Hit
GGTCAAATTCCATTTTTTTGTATACCATTGTGGGATCTTGTGACCATTGA12.1739130434782608No Hit
CTTCAGGTCAATGCTTGCTAGCATTTCTGATGGTATTTGTGTTCGAATCT12.1739130434782608No Hit
GACCTATTGGTCAGAAACAACATCGCCTGACTAAGGGAGGCGATCTTCTA12.1739130434782608No Hit
GGAAGACATAATGACACAATGCTTCAATCCAATGATCGTCGAGCTTGAGG12.1739130434782608No Hit
CGACTTAGTTCTTCACTGGACGAACAGATCTTAATGATCTCAGACAACTC12.1739130434782608No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTAATATAAACTG12.1739130434782608No Hit
AGTTAACCCACCAAAAATGAAAGATGAGCTAATCCTCAACCCTAATCCTG12.1739130434782608No Hit
AATCAGTGATGCTGTGCAAGCTATTAGAAGCGGACGTATGTTATAAATTT12.1739130434782608No Hit
CTCTTGTTCTGCTTCAAGCAGGATTGGTAAGGCTTGAATAAATGGAAATT12.1739130434782608No Hit
GTACATGATACAAAGAGAATTTGACCCTAAACCAATGTTTGAACCAGTAC12.1739130434782608No Hit
AGTTGATAGTGAGCGGGAGAGACGAGCAGTCAATTGCTGAGGCAATAATT12.1739130434782608No Hit
GCACCTATATTTTTATATGGAGTAGTTGATGCACAATTGAAGATACATCT12.1739130434782608No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)