FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_A09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_A09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1177159
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT27480.23344340059414234No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG27230.23131964331071672No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA23950.20345594775217282No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG22970.19513081920114445No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA22080.1875702432721493No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20970.17814076093373962No Hit
ATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTACG19770.1679467259732967No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC19360.16446376402847873No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG18860.16021624946162752No Hit
AACTTATACAATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATG18520.1573279395561687No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT18320.15562893372942824No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT18010.1529954746979805No Hit
GTATAACATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGT17740.15070181683188083No Hit
TTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCGAAAAGCTTGA17710.15044696595786974No Hit
CTTCTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCAT16930.14382084323358188No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA16030.1361753170132497No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA15650.13294720594244278No Hit
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT15100.12827493991890646No Hit
CCTATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACATGTT15080.1281050393362324No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG14920.12674583467484002No Hit
GTACTATTGTCTCCCGAAGAAGTCAGTGAAACGCAAGGAACTGAGAAGTT14550.12360267389537012No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC14470.12292307156467394No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA14350.12190366806862964No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG14300.12147891661194452No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA14250.12105416515525941No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG14180.12045951311590022No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA14090.11969496049386702No Hit
GTGTTTATATTGAAGTGTTGCACTTAACCCAAGGGACGTGCTGGGAGCAG13720.11655179971439711No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT13480.11451299272230854No Hit
CATATAGGTGCCATAGGGGAGACACACAAATTCAGACGAGAAGATCATTT12870.10933102495075007No Hit
GGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAC12830.10899122378540196No Hit
ACACTAAGTGGAATGAAAATCAAAATCCTCGAATGTTCCTGGCGATGATT12790.10865142262005388No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG12670.10763201912400958No Hit
ACTTAGTGTTGTCCCCAGTGATTGTGAAAGAAATCTCTGTGTCTTGTGAA12620.10720726766732447No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC12210.10372430572250647No Hit
CTTCATACCCTTCATGTACTCTTATTTTCAGTGTTTGGAGGTTGCCCGTT12140.1031296536831473No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT12090.1027049022264622No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG11850.10066609523437361No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATA100.0070829056145.05553145
GCAGGTA1650.0114.208412
AGTAGAA3350.099.598841
CAGGTAC1900.099.1093443
GGTACTG2050.088.290695
GCCCCCG259.37289E-487.033325145
AGGTACT2450.076.830924
GCCGTCG300.001932888272.52777145
GTAGAAA4950.067.353682
GTATAGC651.393746E-766.954411
GCATTTA350.003561251862.16666145
GACAGTA2300.059.8048027
TTTAAAC5900.057.695333
CAATTCG5700.057.1614659
ACTGGCT651.1003951E-555.7123533
GCAGGGT651.1028906E-555.691054
GCGCATC400.006041988754.395824145
GAGTTAT400.006041988754.395824145
ATATTCA3350.054.028634
GTACTGG553.2118039E-452.7519571
Produced by FastQC (version 0.11.5)