FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_A06-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_A06-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences911586
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGT350.00358132362.0773964
AGCGAAA2050.056.653191
CAATTCG2150.050.525349
CGAAAGC2900.042.469213
GCGAAAG3100.037.4271662
CCTATAC2002.2555469E-1036.223743
TACGCAG2258.021743E-1032.188285
CAGCTCG5400.028.16329
TATACAG6750.026.8235665
GTTACGC2259.1637594E-725.7591043
GTATATA12950.024.6627331
GTATTAT5750.023.9852391
TTACGCA2751.9883555E-723.702284
CTTATGC2151.8759907E-523.587553
TATATAC13650.022.8222833
TAGAAAC8300.022.6943913
TATATTC6450.022.4643343
CTATACT4850.022.3990614
GTATAGA5850.022.3344331
GTATAGG1700.003071166621.349091
Produced by FastQC (version 0.11.5)