FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n02_S1_R1_A05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n02_S1_R1_A05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGAGGAGAGCCGGGAGCCAAAATACCCTGCGACGCAAATGGAACTCGA15.263157894736842No Hit
GGATCACACCGAGTGATCGTATCACCTCTGCCCGTAACATGGTGGAATAG15.263157894736842No Hit
TTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAG15.263157894736842No Hit
GCGCAATGGAGACTCTGCCGCCATGGATGACGTTCAGCAGATCCCAATGA15.263157894736842No Hit
CATTAAATACGATGACCAAAGATGCAGAGAGAGGCAAGTTAAAAAGAAGG15.263157894736842No Hit
CCATATGGCAATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGCACC15.263157894736842No Hit
TACTCCCATTTTGCTGACTCTTATCCCTCTCAGCGACATCTCCGTGCTTG15.263157894736842No Hit
TTCTAATAGATGGAACAGCATCACTGAGTCATGGGATGATGATGGTCATG15.263157894736842No Hit
GTTTCTCAAAGCGAATCTCTGTATATTTTCAGAGACTCGACCCGTGTTAC15.263157894736842No Hit
GTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTC15.263157894736842No Hit
TCGCTGCCCAAAGCCTCCCGCCGAACTTCTCCTGCCTTGAGAATTTTAGA15.263157894736842No Hit
GTGTAATGGATCATGAAAGAGGAAACCATCGAGACACTTAGAATGACAAT15.263157894736842No Hit
GCGTACATGCTAGAAAGAGAATTGGTCCTTAAAACAAGGTTTCTCCCAGT15.263157894736842No Hit
TTGCATGATAAAGGAAGTTAGGGGCGATCTGAACTTTGTCAATAGGGCAA15.263157894736842No Hit
AATACGAATGGCCACACAACAAGTACAGTTCATTAAACAAAGGTATATAA15.263157894736842No Hit
GAATACCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGG15.263157894736842No Hit
ATTGGGAAAGTGTGCAGGACCTTACTGGCAAAATCTGTATTCAACAGTCT15.263157894736842No Hit
CTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAAGTTTTATTATTTG15.263157894736842No Hit
ACGAAATGTAACTTGTAAGTAAACCTAGGGTGTTATCTCGATGGCTGGTG15.263157894736842No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)