FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133233299
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAC93300.045.2283023
CAATTCG133300.043.0114949
GCAGGTA103700.040.975082
TATATTC166950.037.218883
CCTTATA189250.033.7169042
CCCTTAT202400.030.7994231
ACTGATC150200.029.582058
GACAGTA134550.029.090267
GGATAGC112900.028.6396565
TTTAAAC224950.027.461393
ACGGCCG32200.025.667843145
AGGTCGT107750.024.1549346
TATACTG338900.022.8682545
TTCGTGT52550.022.76416145
ATATATT266600.022.2753562
TACTGGA309450.022.1349687
ACAAGGT202200.021.472537
TCTAAGG91200.021.3874172
GGTCGTT121900.020.870897
ATATAGT113950.020.2522241
Produced by FastQC (version 0.11.5)