FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_H12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_H12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTTGA27.6923076923076925No Hit
AAAATCTGGTAAATCCTTGTTGATTCCAGCTTTACCCCATCTATTTCTTC13.8461538461538463No Hit
GGCTGGTGTTGAGAATAGCACGATTACTCTTGTGACTCCAGTAATGTGTA13.8461538461538463No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAACTAGGTCATTAC13.8461538461538463No Hit
ATATGTGGCCTTCCCTCCGTGTTACTCCAAATTGAATGAACCGGTTCTAG13.8461538461538463No Hit
CAATCTCCTGGTTGCCTTTCTGAGAATAGCTGTTGCTCTTCTCCCAACCA13.8461538461538463No Hit
CACTGTTCCAGCAAATTCGGGATGTGCTTGTGACATTTGACACTGTCCAA13.8461538461538463No Hit
ATGACACTAATAAAGAGATATCACGCAATATATCGGCGATATCACCGAAG13.8461538461538463No Hit
TAGCATTTCTGCTGGTATTTGTGTTCGAATCTTCATTCTTTTACTCTCGA13.8461538461538463No Hit
CTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAA13.8461538461538463No Hit
GATGAGTCCCAATAGTTCTCATTGCATGTACCAGCTGCCTAGTCTGAATA13.8461538461538463No Hit
GTGGTATTCACTCCCCCTCGTGCCTCGGGGGATCAATAATTAATTTAAGA13.8461538461538463No Hit
GCCAAGTACAAAGAACATGAATAGAAAAAGCAAATTGAAGTGGGAACTCG13.8461538461538463No Hit
ATCCTAATTCGGGAGACCTTGAGGGTGGTGATCGCCCCTTGTGTTTTGAA13.8461538461538463No Hit
CCCCTATTGCTGCCTTGCACATGTCTACGGCGTGTTCCTCAGTTGGATTC13.8461538461538463No Hit
CGAGGATAAGCGGAAGGATGTGCTACAGGTTAGTCGTTGGGGAGTCATTT13.8461538461538463No Hit
GTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTAACAGTGATAA13.8461538461538463No Hit
GTTTAAAATGAATCCAAACCAAAAGATAATAACCATTGGTTCGGTCTGTA13.8461538461538463No Hit
CATTGTCGATGGATTCAATTCCCCAGTTCTGGAAAAGCACTTTTGCATCT13.8461538461538463No Hit
AGCCCGGAGATGTCGCTGAGAGGGATAAGAGTCAGCAAAATGGGAGTAGA13.8461538461538463No Hit
GTATTGGAGTAACAGTGATAAAGAACAACATGATAAACAATGACCTTGGA13.8461538461538463No Hit
GCACAGATCTTCATGCTCTCAGAGCACTCTTCTGTCATGGTCGGTCCAGC13.8461538461538463No Hit
ACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTCTTCTA13.8461538461538463No Hit
ATCGCAACACCTGGGATGCAGATTATAGGATTCCTAAACTTAGTTGAAAC13.8461538461538463No Hit
GGGAGCAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTG13.8461538461538463No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)