FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_H11-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_H11-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1413236
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTATATAGTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCA41030.29032659796382204No Hit
ATATAGTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCATCAAC34470.24390830689283316No Hit
TCATAAGACAGAGCAACCTACTGTCCTCTATCCACAATTCCAAATTTTAA21990.15560033851387878No Hit
GTCAAGAGGGTGCGCGGGCCGGAAGAACACCGGGAGGCAGCCGGAACAGC19600.13868879649258864No Hit
TAGATATGCTCTGTACCTGGGTTTCCTCACTAATAAAATGGGGCTCATAA18910.13380638477932916No Hit
ATATAGTACAGGCACCATAGTCCTCTTCTAAAATCCAGCAAATTCCAAAT17290.1223433311916764No Hit
GTATAAATATTTACCCCAAGAAAATAGTATGTTCTGTCACATAGCTGAGG15590.11031420088364577No Hit
GTACATATGATCCCCAGTAGGATACCATTGTCTTAATGCATCAACATTCC14750.10437039531967768No Hit
GTCTTAATGCATCAACATTCCTGCAGTCAAATGTTTGAATCCTTGTATCA14750.10437039531967768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3000.0108.775751
AGTACGT1800.076.524238
GGTGCGC5400.061.7563939
AACGTTC502.0109904E-457.997317
CGAAAGC6150.053.0575833
GCGAAAG6400.050.985022
GTACGTA3200.043.044889
TTGCGCA1703.8198777E-1142.652626145
GTAGTAC2050.042.4460643
AAGTACG3600.038.2621157
TACTATA21550.037.3495832
ATTTACC7550.036.4883779
GTACTAT23100.035.4737631
ATTACGG2103.783498E-1034.529545
AAGTGCG1905.4315024E-934.340518
ACTAGTC1707.815288E-834.1233063
CATTACG2206.220944E-1032.9600144
GGGTAGT3350.032.470371
ACTATTG3850.032.0115058
ACTATAT25200.031.9398213
Produced by FastQC (version 0.11.5)