FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_H10-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_H10-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503046
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGA42630.8474374112904187No Hit
TAATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTG30680.6098845831196351No Hit
CCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATAT25470.5063155258167246No Hit
AAAGCAGGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGC24800.49299666432095673No Hit
GAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGCTATGC22390.44508852073170246No Hit
GAAATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTG19500.3876385062201071No Hit
ATTATATACTGACACACACACACACACACACACAGTACACCAGAGCTATA18990.3775002683651197No Hit
GTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTGCT18060.35901289345308385No Hit
GGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCC17790.35364559105926696No Hit
CCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGTCACGGTGACGTAGAC17030.3385376287655602No Hit
CACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATC15120.30056893405374463No Hit
GTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGCTATGCTTAGG14290.28406944891719643No Hit
TCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTACAAGGC14250.28327429300700135No Hit
CTCTCTGCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTCCGTC13690.27214211026427004No Hit
GTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATT13310.2645881291174167No Hit
CCTATAGGGAAAGGAGACAGCGGGGCAGCCTTCAATTGAAACTGGGGAGA13260.26359418422967285No Hit
CCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGTCACG12370.24590196522783206No Hit
CAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCACACAAAC12130.2411310297666615No Hit
GGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGC11590.2303964249790278No Hit
GGGTTGGGGTGTTGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAG11510.2288061131586376No Hit
ATTTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGG10770.21409572882002836No Hit
GCTTTAGCATCTCTGGGCCTCAATTTCATAATCTGTAAATTAGGCATAAT10720.21310178393228454No Hit
GAGCTATACATTGTGCTCACTAGAACACCATCACACAAACACATCAAAGG10350.20574659176297994No Hit
GCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTACAAGGCTAGTCTT9740.19362046413250478No Hit
GTACAACACCATGCTGGGCTACAAGAGGCACCTGGCCCTCCATGCGGCCA9710.19302409719985847No Hit
GCTATACATTGTGCTCACTAGAACACCATCACACAAACACATCAAAGGGC9510.18904831764888302No Hit
GCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTCCGTCTCGGAA9480.1884519507162367No Hit
TCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGATGATAGTGCTGG9270.18427738218771245No Hit
ATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTGTG9030.17950644672654192No Hit
GTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCACACAAACAC8980.17851250183879805No Hit
GGGTTGGGGTTGGGGTGTTGTCAGTTCTTCAGTTATTATGTCATCTTATC8820.17533187819801768No Hit
GTATAGAAGCATCTCTCACAGTGGTCGCACTGGTGCTTCTTTTCCTTGGT8790.17473551126537135No Hit
GTCCTGGATGACCTCTTGGGAAGTTACCCAGCTTTCTTTCTGTGGTACAG8550.16996457580420082No Hit
CTCTCACAGTGGTCGCACTGGTGCTTCTTTTCCTTGGTTCCGCTCTGGGG8530.16956699784910326No Hit
GGGTAGGAGCGGTGGCCACTTCCCTCAGAAACTGTCGATGCCTCTCAGAA8450.1679766860287131No Hit
AAATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGG8170.16241059465734745No Hit
GTATAAAGTCCCGCCCAAAACTGTACCACAGAAAGAAAGCTGGGTAACTT8090.16082028283695726No Hit
GTGCAGGAGCTCCTATAGGGAAAGGAGACAGCGGGGCAGCCTTCAATTGA8090.16082028283695726No Hit
GTTGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGA8050.16002512692676216No Hit
CTAGAACACCATCACACAAACACATCAAAGGGCAGGATCATGGCATTTGA8040.1598263379492134No Hit
CACCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAAT8020.15942875999411585No Hit
AGCGAAAGCAGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATA7850.1560493473757867No Hit
GTATGCGCCCCAGCTTGAAGCCCCTTAAATCGACAAACTCCTGCATGAGC7850.1560493473757867No Hit
GTGTCCAACTGGGCAGCCAGTAAACACACCTATTACTGTTATGATCATTC7830.15565176942068917No Hit
GGATTGGGTGCATTTAAAAGGGTAATATGTGTGAATCCCTTGGGACAACA7810.1552541914655916No Hit
ATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAG7790.15485661351049407No Hit
CACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCA7740.15386266862275022No Hit
CCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTAC7490.14889294418403087No Hit
CTCTTTCATCAGCTCCTGTGAGTGTGTCTTCTTGGTATGTCGAGTGAGGT7360.14630868747589684No Hit
GCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCC7350.14610989849834807No Hit
CTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATTGGA7320.14551353156570174No Hit
GTACAGAGTCGGGGAGATCGGGGAGAACAGCGGGGTTTTCCAGAGCGGTC7110.14133896303717752No Hit
ATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTGCTACAAATAGT7090.14094138508207996No Hit
CTCTCTCTCTGCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTC7080.14074259610453121No Hit
CTCAAAGATGATTATATACTGACACACACACACACACACACACAGTACAC7010.13935107326168977No Hit
TCTCTGGGCCTCAATTTCATAATCTGTAAATTAGGCATAATAGTACCTAG6970.1385559173514947No Hit
CTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGGGTGCATTTAA6820.1355740826882631No Hit
GACAAAAGAGAGAGTGAGAGTCGGAACTAGTGGAGGGGTTGTGCCTCTCC6740.13398377086787291No Hit
TGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACA6720.13358619291277538No Hit
GTAAACACACCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGG6660.13239345904748273No Hit
AGCGAAAGCAGGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCA6620.13159830313728765No Hit
CCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTAC6550.13020678029444624No Hit
GTGTTGTACTTCTTCCCACACTCCTCACACCCAAAGGCCATCTTGTTGGG6520.12961041336179993No Hit
CTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGC6470.12861646847405606No Hit
TTATACTGCAGTGGAAGTGATGCTGAGACCACGGGCATCTGCGAGGGGAA6360.12642978972101954No Hit
CCTCAAAGCCCATGCTGAGGAAAAGCCCCAGAGCGGAACCAAGGAAAAGA6310.1254358448332757No Hit
GACGTAGACCTTGAGTCACGCTCCGTCTCGGAACAGTGACCAGATCTTGT6270.1246406889230806No Hit
TCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCAT6180.12285158812514163No Hit
ACTTTATACTGCAGTGGAAGTGATGCTGAGACCACGGGCATCTGCGAGGG6150.12225522119249531No Hit
GTATTATTGGGCAAAAATATGCTATTGCTACAAATAGTAACACTCTTCAC6080.12086369834965391No Hit
TTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGGGT6040.12006854243945882No Hit
GCATTTGGTCAGCGCATCTGAAAAGGAAGGTGTGAGAAGTAAAGCCCATG6030.11986975346191003No Hit
ATCCAGGACTGCGGACACCACCGTGAGCCATCCTGGCCCCTCGGGTTCTG5980.11887580857416619No Hit
TCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGG5910.11748428573132477No Hit
GTGCAGGGACAGTCCGAGGTGCTTCCTGGGGAAAGAGAAAGGTGAACAAA5890.11708670777622722No Hit
TCACAGCACAGTGTCCAACTGGGCAGCCAGTAAACACACCTATTACTGTT5880.11688791879867846No Hit
CACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCAC5880.11688791879867846No Hit
GTGAAAGGGAAAGGGAGTGGGTTGGGGTTGGGGTGTTGTCAGTTCTTCAG5850.11629155186603213No Hit
ATATTACCCTGCATCTAATGTACTTGAATTCCAGCAAACTGCTAAGGCCA5780.11490002902319071No Hit
GTTTTAGGATGGGGAGACCCAGACTCTGGGTGTCAGACTGAGGGAGGGAG5780.11490002902319071No Hit
CTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTG5770.11470124004564196No Hit
CACACACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAAC5680.11291213924770299No Hit
CCCTGGAGAAGTTCACTATCCACAATTATTCCCACTCCAGGGAGCGACCT5620.11171940538241036No Hit
CACTAGAACACCATCACACAAACACATCAAAGGGCAGGATCATGGCATTT5620.11171940538241036No Hit
CAATTTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATT5530.1099303045844714No Hit
GCTCAGCACCATCCTTCCATTTGTGCTTAAATGGCACAGCATTTGGTCAG5400.10734604787633736No Hit
CTCCTACCCCGCCCTGCACTCCTCACGGCGGCGACCGCTCTGGAAAACCC5400.10734604787633736No Hit
TTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTA5380.10694846992123981No Hit
AGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTC5270.1047617911682033No Hit
ATATTTGGTATTATTGGGCAAAAATATGCTATTGCTACAAATAGTAACAC5270.1047617911682033No Hit
CACACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACAC5250.10436421321310577No Hit
CATCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGATGATAGTGCT5230.10396663525800821No Hit
GCTGTGGGGAGTGAAAGGGAAAGGGAGTGGGTTGGGGTTGGGGTGTTGTC5190.10317147934781312No Hit
TGGCTACCCATTCGCCCCAGAAATCTCACCAGTGTGCTCACTGTGAGAAG5170.10277390139271558No Hit
AGCAGGAGGTGACAACATCACTCACTCAAAGATGATTATATACTGACACA5160.1025751124151668No Hit
ACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTGTGAATCC5150.10237632343761804No Hit
GAACTGGCACAGGAAGTCCTTGCAGCCGGTGTGGACCACCAGGTGGCGCC5140.10217753446006925No Hit
AAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCAT5130.10197874548252049No Hit
CTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATG5110.10158116752742295No Hit
CTCATAAGGTACATTATTTCCCAAAAACTATACCCCAATATTACCCTGCA5110.10158116752742295No Hit
CTCCAGGGAGCGACCTTACAAGTGCTTGCAGCCAGACTGTGGCAAAGCCT5090.1011835895723254No Hit
GGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCAC5090.1011835895723254No Hit
CCCTAGCTCCAGGGCACAGACCCCACAGGTGAGGTCGCCGCTGCTGGCCG5080.10098480059477662No Hit
ATTATATACTGACACACACACACACACACACAGTACACCAGAGCTATACA5050.10038843366213031No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGCC151.2292349E-4145.01213145
AGCGAAA4100.0114.97151
CTAACGT259.3734905E-487.0245744
CGAAAGC5600.085.4705663
GCGAAAG5550.084.933912
TAACGTG300.001933013172.5204855
CCATAGA300.001933013172.5204852
CTGTCGG5900.063.8972979
AGCAGGC6600.062.6313253
GAGCGAA350.003561485362.160411
GAAAGCA7950.061.1178934
CTCCGAC400.006047142754.37955145
AAACGAC805.868442E-754.3741469
GAAAACT5800.053.765193
TTAGCAT3200.052.1240964
TATGCGC2250.051.570122
AGGCTGT7700.048.9748736
GTTACGG450.00962562548.3469851
AAGCAGC450.00962562548.3469851
TTACGGG450.00962562548.3469852
Produced by FastQC (version 0.11.5)