FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_H08_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_H08_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496283
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGA42320.8527392636862436No Hit
TAATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTG30340.6113447367731718No Hit
CCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATAT25110.5059613164263131No Hit
AAAGCAGGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGC24630.496289415514938No Hit
GAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGCTATGC22180.446922421279794No Hit
GAAATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTG19260.3880850240689284No Hit
ATTATATACTGACACACACACACACACACACACAGTACACCAGAGCTATA18830.3794206128358215No Hit
GTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTGCT17860.3598753130774175No Hit
GGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCC17580.35423337087911533No Hit
CCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGTCACGGTGACGTAGAC16880.3401285153833599No Hit
CACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATC14970.30164240967351286No Hit
TCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTACAAGGC14150.28511957894991363No Hit
GTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGCTATGCTTAGG14120.2845150851429527No Hit
CTCTCTGCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTCCGTC13590.2738356945533093No Hit
GTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATT13260.2671862626767389No Hit
CCTATAGGGAAAGGAGACAGCGGGGCAGCCTTCAATTGAAACTGGGGAGA13180.26557427919150967No Hit
CCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGCGTCACG12280.24743946498268127No Hit
CAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCACACAAAC12040.24260351452699366No Hit
GGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCATATCGC11520.23212562187300392No Hit
GGGTTGGGGTGTTGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAG11410.2299091445808138No Hit
ATTTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGG10710.21580428908505833No Hit
GCTTTAGCATCTCTGGGCCTCAATTTCATAATCTGTAAATTAGGCATAAT10630.21419230559982916No Hit
GAGCTATACATTGTGCTCACTAGAACACCATCACACAAACACATCAAAGG10270.20693837991629777No Hit
GCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTACAAGGCTAGTCTT9690.1952514996483861No Hit
GTACAACACCATGCTGGGCTACAAGAGGCACCTGGCCCTCCATGCGGCCA9620.19384101409881055No Hit
GCTATACATTGTGCTCACTAGAACACCATCACACAAACACATCAAAGGGC9460.19061704712835217No Hit
GCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTCCGTCTCGGAA9380.18900506364312297No Hit
TCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGATGATAGTGCTGG9190.18517660286570364No Hit
ATAGTACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTGTG8960.1805421503456697No Hit
GTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCACACAAACAC8930.17993765653870875No Hit
GGGTTGGGGTTGGGGTGTTGTCAGTTCTTCAGTTATTATGTCATCTTATC8740.17610919576128942No Hit
GTATAGAAGCATCTCTCACAGTGGTCGCACTGGTGCTTCTTTTCCTTGGT8710.1755047019543285No Hit
GTCCTGGATGACCTCTTGGGAAGTTACCCAGCTTTCTTTCTGTGGTACAG8510.1714747432412555No Hit
CTCTCACAGTGGTCGCACTGGTGCTTCTTTTCCTTGGTTCCGCTCTGGGG8430.1698627597560263No Hit
GGGTAGGAGCGGTGGCCACTTCCCTCAGAAACTGTCGATGCCTCTCAGAA8380.16885527007775805No Hit
AAATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGG8100.1632133278794559No Hit
GTATAAAGTCCCGCCCAAAACTGTACCACAGAAAGAAAGCTGGGTAACTT8060.16240733613684127No Hit
CTAGAACACCATCACACAAACACATCAAAGGGCAGGATCATGGCATTTGA8020.1616013443942267No Hit
GTTGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGA7980.1607953526516121No Hit
GTGCAGGAGCTCCTATAGGGAAAGGAGACAGCGGGGCAGCCTTCAATTGA7960.16039235678030478No Hit
CACCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAAT7920.1595863650376902No Hit
GTATGCGCCCCAGCTTGAAGCCCCTTAAATCGACAAACTCCTGCATGAGC7780.1567653939385391No Hit
AGCGAAAGCAGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATA7770.15656389600288545No Hit
GGATTGGGTGCATTTAAAAGGGTAATATGTGTGAATCCCTTGGGACAACA7770.15656389600288545No Hit
GTGTCCAACTGGGCAGCCAGTAAACACACCTATTACTGTTATGATCATTC7750.15616090013157816No Hit
ATAAAACTCTTCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAG7700.1551534104533099No Hit
CACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCA7690.15495191251765625No Hit
CCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCATTAC7370.14850397857673947No Hit
CTCTTTCATCAGCTCCTGTGAGTGTGTCTTCTTGGTATGTCGAGTGAGGT7340.14789948476977854No Hit
CTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACAGAATTGGA7270.146488999220203No Hit
GCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCC7240.14588450541324205No Hit
ATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTGCTACAAATAGT7080.14266053844278367No Hit
CTCTCTCTCTGCCTACGGCGTCACGGTGACGTAGACCTTGAGTCACGCTC7070.14245904050712999No Hit
GTACAGAGTCGGGGAGATCGGGGAGAACAGCGGGGTTTTCCAGAGCGGTC7070.14245904050712999No Hit
CTCAAAGATGATTATATACTGACACACACACACACACACACACAGTACAC6940.13983956734363256No Hit
TCTCTGGGCCTCAATTTCATAATCTGTAAATTAGGCATAATAGTACCTAG6940.13983956734363256No Hit
CTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGGGTGCATTTAA6740.13580960863055958No Hit
TGTCAGTTCTTCAGTTATTATGTCATCTTATCAGTCAGCACATGCTGACA6660.13419762514533037No Hit
GACAAAAGAGAGAGTGAGAGTCGGAACTAGTGGAGGGGTTGTGCCTCTCC6630.13359313133836945No Hit
GTAAACACACCTATTACTGTTATGATCATTCAATATTTGGTATTATTGGG6580.1325856416601012No Hit
AGCGAAAGCAGGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCA6560.13218264578879388No Hit
CCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTAC6470.13036916436791104No Hit
GTGTTGTACTTCTTCCCACACTCCTCACACCCAAAGGCCATCTTGTTGGG6460.1301676664322574No Hit
CTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGGC6420.1293616746896428No Hit
TTATACTGCAGTGGAAGTGATGCTGAGACCACGGGCATCTGCGAGGGGAA6280.12654070359049172No Hit
CCTCAAAGCCCATGCTGAGGAAAAGCCCCAGAGCGGAACCAAGGAAAAGA6220.1253317159765698No Hit
GACGTAGACCTTGAGTCACGCTCCGTCTCGGAACAGTGACCAGATCTTGT6200.12492872010526253No Hit
TCCCCATATCGCTATGCTTAGGAAACTGAAGGGTCTGGAGATAAATTCAT6100.12291374074872602No Hit
ACTTTATACTGCAGTGGAAGTGATGCTGAGACCACGGGCATCTGCGAGGG6070.12230924694176509No Hit
GTATTATTGGGCAAAAATATGCTATTGCTACAAATAGTAACACTCTTCAC6010.12110025932784318No Hit
TTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATTGGGT5990.12069726345653589No Hit
GCATTTGGTCAGCGCATCTGAAAAGGAAGGTGTGAGAAGTAAAGCCCATG5950.11989127171392129No Hit
ATCCAGGACTGCGGACACCACCGTGAGCCATCCTGGCCCCTCGGGTTCTG5930.119488275842614No Hit
TCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCACTCTCTCTCTGCCTACGG5890.1186822840999994No Hit
TCACAGCACAGTGTCCAACTGGGCAGCCAGTAAACACACCTATTACTGTT5830.1174732964860775No Hit
CACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACACCATCAC5810.11707030061477021No Hit
GTGCAGGGACAGTCCGAGGTGCTTCCTGGGGAAAGAGAAAGGTGAACAAA5790.11666730474346289No Hit
GTGAAAGGGAAAGGGAGTGGGTTGGGGTTGGGGTGTTGTCAGTTCTTCAG5780.11646580680780924No Hit
GTTTTAGGATGGGGAGACCCAGACTCTGGGTGTCAGACTGAGGGAGGGAG5760.11606281093650196No Hit
CTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTATTG5740.11565981506519467No Hit
ATATTACCCTGCATCTAATGTACTTGAATTCCAGCAAACTGCTAAGGCCA5720.11525681919388735No Hit
CACACACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAAC5650.1138463336443118No Hit
CACTAGAACACCATCACACAAACACATCAAAGGGCAGGATCATGGCATTT5620.11324183983735087No Hit
CCCTGGAGAAGTTCACTATCCACAATTATTCCCACTCCAGGGAGCGACCT5560.11203285222342897No Hit
CAATTTCATAATCTGTAAATTAGGCATAATAGTACCTAGCACACAGGATT5480.11042086873819978No Hit
GCTCAGCACCATCCTTCCATTTGTGCTTAAATGGCACAGCATTTGGTCAG5380.10840588938166329No Hit
TTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATGCTA5370.10820439144600964No Hit
CTCCTACCCCGCCCTGCACTCCTCACGGCGGCGACCGCTCTGGAAAACCC5350.10780139557470235No Hit
AGGGAAAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTC5230.10538342034685855No Hit
ATATTTGGTATTATTGGGCAAAAATATGCTATTGCTACAAATAGTAACAC5200.1047789265398976No Hit
CACACACACACACAGTACACCAGAGCTATACATTGTGCTCACTAGAACAC5200.1047789265398976No Hit
CATCTTATCAGTCAGCACATGCTGACAGAATTGGAAATGATGATAGTGCT5190.10457742860424395No Hit
GCTGTGGGGAGTGAAAGGGAAAGGGAGTGGGTTGGGGTTGGGGTGTTGTC5130.10336844099032205No Hit
TGGCTACCCATTCGCCCCAGAAATCTCACCAGTGTGCTCACTGTGAGAAG5120.1031669430546684No Hit
GAACTGGCACAGGAAGTCCTTGCAGCCGGTGTGGACCACCAGGTGGCGCC5090.10256244924770745No Hit
ACCTAGCACACAGGATTGGGTGCATTTAAAAGGGTAATATGTGTGAATCC5090.10256244924770745No Hit
CTCATAAGGTACATTATTTCCCAAAAACTATACCCCAATATTACCCTGCA5090.10256244924770745No Hit
GGCTGTCGGTCGGCTCCTCTCTCTCCCCTCAGCCTCCTCAAAGCTCCCAC5080.1023609513120538No Hit
AAACTGGTCTGAAAAGTTCTGTGTCCCTCTGAAATAAAACTCTTCCCCAT5070.10215945337640016No Hit
CCCTAGCTCCAGGGCACAGACCCCACAGGTGAGGTCGCCGCTGCTGGCCG5050.10175645750509286No Hit
AGCAGGAGGTGACAACATCACTCACTCAAAGATGATTATATACTGACACA5040.10155495956943922No Hit
CTATTACTGTTATGATCATTCAATATTTGGTATTATTGGGCAAAAATATG5030.10135346163378557No Hit
CTCCAGGGAGCGACCTTACAAGTGCTTGCAGCCAGACTGTGGCAAAGCCT5010.10095046576247826No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGTT100.007083476145.04324145
ATCAGTT202.1263804E-6145.04324145
GTGACTA100.007083476145.04324145
AGCGAAA4100.0107.8980261
GAGCGAA406.1020546E-790.652021
GTACAAG259.3728304E-487.025953
GCGAAAG5250.084.2632142
CTCCGAC353.430488E-582.88186145
CGAAAGC5450.081.170993
GGTAGCT1200.066.4513557
AGCAGGC5900.065.1465453
AGGCTGT5800.065.019396
ATATGCA451.1915082E-464.463664
TATGCAA451.1915082E-464.463665
CTGTCGG5850.064.437689
GAAACGC606.788545E-660.434686
GAAAGCA7600.059.1623734
TAGCTTT1350.059.067889
TTAGCAT3000.058.0172964
TGAAACG651.0909083E-555.7858625
Produced by FastQC (version 0.11.5)