FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_H05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_H05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCAAGAGGTTCTGTGATGCTGGGCAATTCATCTAAGCCTTAATTTGCT14.545454545454546No Hit
TTCCCCTTTGGCTTGTGTTGAGAATAGAACGATTCCTCTTGGGAATCCAG14.545454545454546No Hit
CTTCAGGTCAATCCTTGCAAGCATAACCGCGGAAAATTGTGTTCGAAACT14.545454545454546No Hit
GACTTAGACAGAAACTCTCACACAAACATAGACAGTGTCTCACTCAGTTA14.545454545454546No Hit
AATGTGCAATATCCTCAAAGGAAAAATTCAAACAGCTGCCCAGAGGGCAA14.545454545454546No Hit
TTATTTGGACAGTGTCAAATGTCCCACGCACATCCCGCATTTGCTGGAAC14.545454545454546No Hit
ATATAAATAACAACCGGTCTCAGAGATTACATGATAGGCTAATAATTATA14.545454545454546No Hit
GCCAAATATCTCATCACAGAAAGACACTGTACAGGAATGATGAATTGATG14.545454545454546No Hit
CATTTATTGCCTAAGCAATTGACTGCTCGTCTCAACCGCTTACAATCAAC14.545454545454546No Hit
GTCATTGATTATCATGTTGTTCTTTATCACTGTTCCTCCACTACTCATGT14.545454545454546No Hit
GGACCCCTTCCCAAGCCAGTTCTCCTGGGACACCTCTCCGCGCGACCTTA14.545454545454546No Hit
ATGTGTGTGTCTGAACGCTTCCACAGAGGTGGAAGGCCTCCCTTGGTTTT14.545454545454546No Hit
TCCTTTTATAATGAACCCATACAGGTTTGTTTTCCGTCTTCCTTCTTTGG14.545454545454546No Hit
GAGTAATAGTAGCGTGTGGGTCTGTGTGTCTGTGTGTCAGTTATCAGATT14.545454545454546No Hit
ATATAGAAGTATCTGCTAACATACTCTGAAAACTGGAATTACCATTGGCA14.545454545454546No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTACTTTCTATC14.545454545454546No Hit
GAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAGGAACA14.545454545454546No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG14.545454545454546No Hit
GAAGTTGTTTTCCCAAATGAAGTGGGGGCAAGAATAATGACATCAGAGTC14.545454545454546No Hit
GTGCGCAGGAGGGAGAGAGCGCGGGTGGGCGGGAGAAGTTTATTGGAGCG14.545454545454546No Hit
GGATGCACAGAAAACTCTTAAAGGCAGAGCCTAACGCCGAAAGATACCAT14.545454545454546No Hit
GTTTGGTCGGCCATGTGATACTTGTACCCCTACGTGTGATTGGTTTTGGG14.545454545454546No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)