FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_H04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_H04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTGATCGGCTCCGCCGAGCTCAAAAGTCCTTAAAAGGAAGAGGCAACACC11.0869565217391304No Hit
GACAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGACAACAAGGCAA11.0869565217391304No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCATATCTCTTG11.0869565217391304No Hit
GACTAGCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAAT11.0869565217391304No Hit
ATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAAT11.0869565217391304No Hit
GCACCAAACGATCATATGAACAAATGGAGACTGGTGGGGAGCGCCAGGAT11.0869565217391304No Hit
CCTAAATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCACCAATTCT11.0869565217391304No Hit
GCACTATTGAAGCACCGATTTGAGATAATTGAAGGAAGAGACCGAATCAT11.0869565217391304No Hit
GAATCAATCTCTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCT11.0869565217391304No Hit
CTTATCAATGGATAATCAGGAACTGGGAAATTGTGAAAATTCAATGGTCA11.0869565217391304No Hit
AGCAAATGTAGGACCAAAGAAGGAAGCCGGAAAACAAACCTGTATGGGTT11.0869565217391304No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT11.0869565217391304No Hit
CTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGCCTCTCCGATTGGC11.0869565217391304No Hit
TGGCTGGACCATGGGCTGGCATTACCACAGCATTGTTTACAGAATCAATC11.0869565217391304No Hit
CTGGCACACCACAGGGGTCGAGCAGCCGCAATTTCGTCCTGCGTTGTATG11.0869565217391304No Hit
GCCGTATTTATGTACACTCCCTTCATTATGTATTCAGTAGCCCTGCAGTG11.0869565217391304No Hit
CAATGAACAAAGAGGAAATAGAGATAACAACCCAGTGTCAAAGAACAGGG11.0869565217391304No Hit
GCTCGAATCCATCTACATAGGCTCTAAAGTTTTCAAGGCTGGGGAAGTTC11.0869565217391304No Hit
GCAATGGCCAGTATGAGTCTATGGCATTCCTTTCGTCAGTCCGAAAGAGG11.0869565217391304No Hit
GAATGAAAAGATGTGAATGTGTGTCTTCTCAGATTTTATTTTGTTGGCTT11.0869565217391304No Hit
GTTAAAAAGAAGGGCTATCGCAACACCTGGGATGCAGATTAGAGGTTTCG11.0869565217391304No Hit
CTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGA11.0869565217391304No Hit
CAATGGAAGTGGTTCACCAAACCAGGGGAGATAAACTACCTCAAGGTCGC11.0869565217391304No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11.0869565217391304No Hit
GGAGTGGAGTAACAGTGATAACGCCCCACCTGATAAACAATGCACTTTTA11.0869565217391304No Hit
AGAGAGCAGGGCAAGAATCAAAACTAGGCTTTTCACTATAAGACAAGAAA11.0869565217391304No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC11.0869565217391304No Hit
GAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTGATGGTGGCGTACA11.0869565217391304No Hit
GATAGCAAATAGTAGCATTGCCACAACTCCTTCAGTGCATGTGTGAGGAA11.0869565217391304No Hit
GCACCCATAATGTTCTCAAACAAAATGGCAAGACTAGGGAAAGGGTACAT11.0869565217391304No Hit
CGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCT11.0869565217391304No Hit
GGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCG11.0869565217391304No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA11.0869565217391304No Hit
ATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAA11.0869565217391304No Hit
GCTAATAGATTTCTTAAAGGATGTAATGGAATCAATGAACAAAGAGGAAA11.0869565217391304No Hit
CTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAGGGAAAAATTTCTC11.0869565217391304No Hit
CCAATTTACAAATCCATAAATAAAAATTACCTCAAGACTTGGAAGCATCT11.0869565217391304No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG11.0869565217391304No Hit
CTTCATCAAGGGTGTAGTCCGCTTTGGTGGCCGTCTCCTCTCCAGTGAAT11.0869565217391304No Hit
CCCCTATGGCACCTATATGTGTATCTGTAGGCGTTGATGAACAAGTGCAG11.0869565217391304No Hit
AAACAATAAGGGCAAGATTAACAAGATCTACAATCGGAGTAAGACGCTCA11.0869565217391304No Hit
GCTCAAAAGAGAAATAACGTTCCATGGGGCCAAGGAGGTGTCACTAAGCT11.0869565217391304No Hit
GGGGCGTAGGCCAGTGCTGCGCCCGCGCCCAGATCTCTAGCAAGCTGGGG11.0869565217391304No Hit
CACCAATACTCAGAAAAGGGAAAGTGGACGACAAACACAGAGACTGGTGC11.0869565217391304No Hit
CTTTATGACTGACAAAGGGATTCAGGGGATTACAAAGTCTTCCCCGATAA11.0869565217391304No Hit
TTCCATCATTCTTATAACTTCTGTTCGCATGTCGGATGGCCGTCGTTCAT11.0869565217391304No Hit
GTTCAACCACCTGGAAAAAAGAATAGAGAATTTAAATAAAAAAGTTGATG11.0869565217391304No Hit
GCAAGGAACTGAGAAGTTGACAATACCTTATTCGTCATCAATGATGTGGG11.0869565217391304No Hit
AGTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAG11.0869565217391304No Hit
GACCAAAGTTTGATTATCGCTGCTAGAAACATAGTAAGAAGAGCAGCAGT11.0869565217391304No Hit
GGCCAGTGCATTCGGGTCACCAGATTCTACAATTATTGATCCACCCCGTT11.0869565217391304No Hit
TTATAGCACCCTTGGGTGTTTGACAAGATGTATTCAAATCGTGGACTGGT11.0869565217391304No Hit
GGGGTGTGGATACTGGTAAGAGGCAATTCCCCAGTATTCAATTACACCAA11.0869565217391304No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA11.0869565217391304No Hit
AGTCAATATCCTCAACCCTGATCCTCTCACATTCACAGTCAATGAGGAAA11.0869565217391304No Hit
TGCTAAAATTAGCCACAAATCCATAGCGATAAAAAAAGCTTGTGAATTCA11.0869565217391304No Hit
GTGTTCTAGAGGCTATGGCTTTCCTTGAAGAATCCCACCCAGGAATATTT11.0869565217391304No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG11.0869565217391304No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT11.0869565217391304No Hit
TCATTAGGCTATGCTAGAGAAAAATTTGTACAAAAACTTCCATAAGTTCA11.0869565217391304No Hit
CATTCCTTGATCGGCTCCGCCGAGATCAAAAGTCCTTAAAAGGAAGAGGC11.0869565217391304No Hit
TGTCTACGGCTTGTTCCTCAGTTGGATTCTGTCTAAGGATGTCCACCATC11.0869565217391304No Hit
GTATGAGAGTGATGAGCCAGAGCCCAGATCTCAAGCGAGCACGGTCCAAA11.0869565217391304No Hit
CCCATCATCATCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGG11.0869565217391304No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGCTATTCTCACAGGAGGATT11.0869565217391304No Hit
GTATTCAATTACAACAAGGCAACCAAACGACTTGGCCCAGCATTAAGCAT11.0869565217391304No Hit
GCTTTTCGCAAATGCTCCTAGATAAAGTTTAAACAAAGTATACGCAACAT11.0869565217391304No Hit
CTAGCAAGCTGGGTCCAAAATGAATTCAATAAGGCATGTGAATTGACTGC11.0869565217391304No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA11.0869565217391304No Hit
GAATGTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATG11.0869565217391304No Hit
CTTCATTATGTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAAT11.0869565217391304No Hit
ATCCATAGCGATAAAAAAAGCTTTTGAATTCAAATGTCCCTGTCTTATTT11.0869565217391304No Hit
CATTCCTGGATTCCCAAGAGGAATCGTTCTATTCTCAACACAAGCCAAAG11.0869565217391304No Hit
GTGTGCTGTGGCACATTTCCAAGAGAGATGCTAATGGGTCTGCTGACACT11.0869565217391304No Hit
ACTCCAGACACTCCAAAGCTGGGTAGCTCCATGCTAAAATTAGCCACAAA11.0869565217391304No Hit
GACCACTGTGGACCATATGGCCATAATCAAAATGTACACATAACGAATCC11.0869565217391304No Hit
CCTATATGTGAGAACCAATGGAACCTCCAAGATCAAGATGAAATGGGGGA11.0869565217391304No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCTGGGCCAAGTCGTTTG11.0869565217391304No Hit
CTGAACCCCATGCACCAACACTTGAGGCATATCCAAAAAGATGCAAAAGT11.0869565217391304No Hit
GAAGAAGGCAGCGCCTCATTTCCATGCCCCATTTCATCTTGATCTTGGAG11.0869565217391304No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG11.0869565217391304No Hit
CAGCGGGGGCGCTCCAGGCTACCATGAGCCACACCTCCAGCGCGAGAAAA11.0869565217391304No Hit
CTATATGCGCCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAATT11.0869565217391304No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA11.0869565217391304No Hit
GGGACGGCTCCAAAAAGGAAGACTTAGTCCGACAATGCGACACTCCAATG11.0869565217391304No Hit
ATTTGACACTGTCCAAATAATAAACCTTCTCCCCTATGCTGCTGCCCCAC11.0869565217391304No Hit
GTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGC11.0869565217391304No Hit
GAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAA11.0869565217391304No Hit
TGCCTAGGCAAAAGATAATAGGCCCTCTTTGCGTGCGATTGGACCAGGCG11.0869565217391304No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG11.0869565217391304No Hit
GTGGAATAGGAATGGCCCAACAACAAGTACAGTTCATTACCCTAAGGTAT11.0869565217391304No Hit
AATCAAAACTAGGCTTTTCACTATAAGACAAGAAATGGCCAGTAGGAGTC11.0869565217391304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAAGCAC50.0145.09
TATTGAA50.0145.05
TGAAGCA50.0145.08
ATTGAAA50.0145.0145
CACTATT50.0145.02
TTGAAGC50.0145.07
GCACTAT50.0145.01
ACTATTG50.0145.03
CTATTGA50.0145.04
ATTGAAG100.072.56
Produced by FastQC (version 0.11.5)