FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_H03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_H03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCAAGTGGGTATGCACAAACAGACTGTGTTCTAGAGGCTATGGCTTT12.857142857142857No Hit
AGTCACAGCTGGCAATAACAAAAGAGACCAAAGAAGAGCTCCAGGATTGT12.857142857142857No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT12.857142857142857No Hit
CTTGTGAACAGATTGCTGATTCACAGCATCGGTCTCACACAAAGATGGCT12.857142857142857No Hit
AAAAGTGCAAAGTGACCTAGTCTTGCATATTAAACGATAACGGGACAATA12.857142857142857No Hit
CACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATG12.857142857142857No Hit
GCGGAAAAGGCAATGAAAGAATATGGGGAAGATCCGAAAATCGAAACTAA12.857142857142857No Hit
GATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAA12.857142857142857No Hit
TGTTGTCTCTTACTCTCCTTTTTCTTTGAAAGTGGGTTGTTATCTCTATT12.857142857142857No Hit
GCACTGACTCAGGGCCATTGATCTCCCACATCATTGATGACGAATAAGTT12.857142857142857No Hit
TAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATACTC12.857142857142857No Hit
GAATACGTGTAGAAATAAAACTTAGGGCAACTGTAACACAAAGGACAAGA12.857142857142857No Hit
AATATAAACACTGCCTGTTCCGCCGGCTACTGGGAGAAACCTTGTTTTAC12.857142857142857No Hit
GAAGAAATGCGGCACAGATTGAAAGAGACAGGGATTAGTTTCGAACAAAT12.857142857142857No Hit
GTCAAGAAAGAAGAAGAAGTGCTAACGGGCAACCTCCAAACACTGAAAAT12.857142857142857No Hit
ATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTGAGGAAG12.857142857142857No Hit
TTTATGGGTTAGAGATCAAAGAGGGAACGTACTATTGTCTCCCGAAGAAG12.857142857142857No Hit
GGGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAAGGGGGGGC12.857142857142857No Hit
AAAAAAAGCTTGTGAATTCAAATGTCACTGTCTTATTTATATAGGACTTC12.857142857142857No Hit
CCTCGATTGCTTCATATAGCCCCCCAAGATAGAAGGTTCCAGCTTCCAGG12.857142857142857No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAACA12.857142857142857No Hit
AGTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGACAGC12.857142857142857No Hit
CATATAGACTGTTGAATACAGATTTTGCAAGTAAGGTCCTGATCACTTTC12.857142857142857No Hit
ATTCAATATGGAGAGAATAAAAGAAGTGAGAGATCTAATGTCGCAGTCCC12.857142857142857No Hit
CCCATATGCTGGAGATACTCCTTAAAAAAATCTACCAGGAACTCTATAAA12.857142857142857No Hit
GCATGCGGCGCGGTGGTTAGTCTCCCATGGATCAGAAGACACCCTGACCC12.857142857142857No Hit
GGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTTTGCCGTCTGAG12.857142857142857No Hit
GAATAGGAATGGCCCAACAACAAGTACAGTTCATTACACTAAGGTAAAAA12.857142857142857No Hit
GGACAGCAGACTCCACCACAGATGTGCCTTCATCTGGATCTTCAGTCAAT12.857142857142857No Hit
ACTGACTTCTTCGGGAGACAATAGTAAGTTACCTCTTTGATCTCTAACCC12.857142857142857No Hit
GTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTGATCCGTCC12.857142857142857No Hit
GTGTGAGGAAGGAGTTGAACCAAGATGCAGTAAGCAAAACCCAGGGATCA12.857142857142857No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC12.857142857142857No Hit
ATACAAGAAGATCAAAAGAGAAATAACGTTCCATGGGTCCAAGGAGGTGT12.857142857142857No Hit
CACATATAGGTGCCATAGGGGAGACACACAAATTCAGCCGAGCAGATCAT12.857142857142857No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)