FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_H01_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_H01_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACGGAATCTTCACATTCCTGAAGTCTGCTTAAAATGGGAGCTAATG15.555555555555555No Hit
GACTAAGGGAATTTTAGGATTTGTGTTCACGCTCAACGTTCCCAGTTAGC15.555555555555555No Hit
GTAATGAACTGTTCTTGTAGTTCGGCCATTACTATTCAACCATGTTAAGC15.555555555555555No Hit
GATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGG15.555555555555555No Hit
CCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCT15.555555555555555No Hit
AGCGGACTACACCCTTGACGAAGAGAGCAGGGCAAGAATCAAAACTAGTC15.555555555555555No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA15.555555555555555No Hit
AAATAAGGAGGAGAGTTGATACAAACCCTGGCCATGCAGATCTCAGTGCC15.555555555555555No Hit
TATTCCATGCTTTTGGCTGGACCATGGGCTGGCATTACCACAGCATTGTT15.555555555555555No Hit
GGACAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAA15.555555555555555No Hit
GTCATTGTTTATCATGTTGTTCTTTATCACTGTTACTCCAATACTCATGT15.555555555555555No Hit
GTGCATTCACTATGAGTGCAAAATCGTCGGATGATTGGAGCCCATCCCAC15.555555555555555No Hit
TTGATCGGCTACGCCGAGATCAAAAGTACTTAAAAGGAAGAGGAAACACC15.555555555555555No Hit
TATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTGA15.555555555555555No Hit
GTGATGGGAATGATCGGAATACTGCCCGACATGACCCCAAGCACGGAGAT15.555555555555555No Hit
ATACTCACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACAC15.555555555555555No Hit
ATTACCCACCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTT15.555555555555555No Hit
GTACATAAATACGGCCGTGCTCAATGCATCCTGTGCAGCCATGCAGGCAT15.555555555555555No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)