FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_G12_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_G12_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATAGGAATGGCCCACCACCCAGTACAGTTGATTGCGCTAACGGAG15.88235294117647No Hit
AGATAAGACTCATACCTGCTAGCCCCTTAGCAGAAGAAGTACTTACTTGT15.88235294117647No Hit
CTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCTGC15.88235294117647No Hit
GTCAGATTGGCTGAAGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGGT15.88235294117647No Hit
GATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCACTGGGG15.88235294117647No Hit
GAATAAGAGCTTCTATCTAAGAAGAGAAAGCAATTTAAATACGAAGAACA15.88235294117647No Hit
ATCCATTCCGGTGCGAACAAGCGCTCTTGTTCTCTGATATGTGGCATCAT15.88235294117647No Hit
AATCAAATCGTGCACTGGATCTTCAAAGACGATTCCAGCCAGAAGCTTAC15.88235294117647No Hit
GGATTGCGTTCGGCACTCCGAAAGAGGCGAAGAGACAAGTGCAGAAAAAT15.88235294117647No Hit
GACCAGCCCACAGTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCC15.88235294117647No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG15.88235294117647No Hit
GAGTATTGTAGTAACAGTGATAAAGAACAACATGATAAACAATGACATTG15.88235294117647No Hit
GGCAATGAGGAAAATTGAATCCTACTCTGTTCTGGGGGGGCAGCAGCAAA15.88235294117647No Hit
CTCCCCTCACGTGTGGCACTAGGGGTCTTTGGATACTCCGGCGAACTCCG15.88235294117647No Hit
CAAATGTTCCTGTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATT15.88235294117647No Hit
GTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACA15.88235294117647No Hit
ACATTATTTTTCCCATTTGTTTTGATAAATTCTAGGGTCAGTGATTTAGA15.88235294117647No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)