FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_G09-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_G09-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences704077
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGTGTCTACCCATAAGTTAACTTGATTTTGGCAAACTGGGAAAAG12370.17569101106839166No Hit
ATGTATAAGTGTCTACCCATAAGTTAACTTGATTTTGGCAAACTGGGAAA11440.16248222850625713No Hit
GGATACAGCGGTCCACATGAGCGAATTCACAGACAAACCATTCACGAGAT11350.16120395922605057No Hit
GTGTGGGCTCAGGCCCAGGGCAGGTGTCCACCAGGGCACTTCCACCAGGG9300.13208782562134538No Hit
GTCCCAGTCCAGTGCCATAGCTGCTCCTGCTGCAGTACCTGCTGTTGAAA8380.1190210729792338No Hit
CAATAATGCAGCAGGAACTGTTTTCTCTGGGGTTTTTTAACGGGCAAGCC8320.1181688934590961No Hit
GTTTAGGGACTGTCCGAACAATTGCTTTATTTCTAGATAATTACTGTATG8220.11674859425886658No Hit
GCCTTAAGGTGAGCTGTCTGAGGAGGAAGGCGGACGGCAAGGAAAGACAG7780.11049927777785667No Hit
CTACAAGTCTGTGTGTGCTTACAAGGGACCTCTTCTCTCTATTACCTCCA7560.10737461953735175No Hit
CTATTACCTCCAGTCCTAGTTGAGACACACCAGCCACTGGGCAAAGGTCA7210.10240357233654843No Hit
CCTCCAAGTCATGGGGTGAATGTTGGACCCCAGTTTACTAAGAAGGGACT7130.1012673329763648No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGT301.5992264E-596.665489
GCTATCG300.001935597772.4991158
CTATCGA350.003566238662.1420949
GAGTCGC606.8040044E-660.4159289
GCGAAAG3250.060.2428672
CGAAAGC3300.059.3300933
TTAACGC1206.002665E-1154.3859184
GGATACA3500.053.867961
TGTATAC553.2151563E-452.737862
TATGTTA1101.6716513E-952.737866
ATAACCT701.6955913E-551.785088
AGCGGTC3650.051.6432047
TAACGCG1301.3096724E-1050.202395
GATAGTG450.00963842648.332747
AACGCGA1402.7102942E-1046.6165056
ATACTTA803.7290945E-545.32166
GTATACT657.327844E-444.6243483
TGTTATA1002.7332135E-643.4994668
TAGCCTG2050.042.4475485
AGCGAAA4550.041.4368931
Produced by FastQC (version 0.11.5)