FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_G06_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_G06_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAGTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATT90.34937888198757766No Hit
CCACTATATGATGCAATCAAATGCATGAAGACATTCTTTGGCTGGAAAGA80.3105590062111801No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG60.2329192546583851No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG60.2329192546583851No Hit
CATATAGACTGTTGAATACAGATTTTGCCAGTAAGGTCCTGCACACTTTC60.2329192546583851No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA50.19409937888198758No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA50.19409937888198758No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC50.19409937888198758No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA50.19409937888198758No Hit
GTGTAGTTGCAACGGCATCATATTCCATGCTTTTGGCTGGACCATGGGCT50.19409937888198758No Hit
ATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGC50.19409937888198758No Hit
CTCTTGAGGCATTTCCAAAAAGATGCAAAAGTGCTTTTCCAGAACTGGGG40.15527950310559005No Hit
GTGTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTAC40.15527950310559005No Hit
CCCCTGGGTGACTCTCCGATTGGCCATGTTTCCGATTTGTTTTCAAAGAA40.15527950310559005No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG40.15527950310559005No Hit
GTCCTGGGATGATGATGGGCATGTTCAACATGCTAAGTACGGTCTTGGGA40.15527950310559005No Hit
GTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACT40.15527950310559005No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATCTGGG40.15527950310559005No Hit
GTCTAAGGATGTCCACCATCCTTACTCCTCCAATCTGTGTGCTGTGGCAC40.15527950310559005No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG40.15527950310559005No Hit
GTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCC40.15527950310559005No Hit
TGCTGGAACAGTGTCCTTACGAATCCACTGTACCGGCTTCTGGTTGCCTT40.15527950310559005No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC30.11645962732919254No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA30.11645962732919254No Hit
ATAGCATTGTGGGATCTTGTGACCATTGAATTTTCACAATTTCCCAGTTC30.11645962732919254No Hit
GAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGAT30.11645962732919254No Hit
CTCCAATACTCATGTCAGCTGATTCATTTACTCCAGACACTCCAAAGCTG30.11645962732919254No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTTTGCTGCTGCCCCAC30.11645962732919254No Hit
GCTTTGGAGTGTCTGGAGTAAATGAATCAGCTGACATGAGTATTGGAGTA30.11645962732919254No Hit
GTGGTATTCCCTCCCCCTCGTGACTCGGGTCTTCAATACTTAATTTCAGA30.11645962732919254No Hit
GAGTCAGTGCTAGTCAACACTTATCAATGGATAATCAGGAACTGGGAAAT30.11645962732919254No Hit
GGCTCTAAAGTTTTCAAGGCTGGGGAAGTTCGGTGGGAGACTTTGGTCGG30.11645962732919254No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT30.11645962732919254No Hit
GAAGTACTTCAATGAATCAACAAAGAAGAAAATTGAGAAAATAAGGCCTC30.11645962732919254No Hit
GTTCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTT30.11645962732919254No Hit
ACCCTATTGCTGCCTTGCATATGTCTACGGCTTGTTCCTCAGTTGGATTC30.11645962732919254No Hit
CCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGAAG30.11645962732919254No Hit
GTTCCTGTAATCTCAAATTTTTCTTCAATTGTCTCTTCGCCTCTTTCGGA30.11645962732919254No Hit
TCATTGGGATCAGCTGAAAGTCATCCATGGCTGCACAGGATGCATTGAGC30.11645962732919254No Hit
ATTATTGCCTCAGCAATTGACTGCTCGTCTCTCCCGCTTACTATCAACTG30.11645962732919254No Hit
GTCTTATTTATATAGGACTTCTTTTTGCTCATGTTGATTCCCACTAACTT30.11645962732919254No Hit
GGGAAAGCTTGCCCTCAATGCAGCCGTTCGGCTCGAATCCATCTACATAG30.11645962732919254No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG30.11645962732919254No Hit
CCTCAAGAGCATGTCTCCTATTTCAAGAACACAGTATTTTTCCCATTTGT30.11645962732919254No Hit
CAATAGTACGTTCCCTCTTTGATCTCTAACCCTTAAAAATCGGTCAATAC30.11645962732919254No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA30.11645962732919254No Hit
GTCAATTGCTGAGGCAATAATTGTGGCCATGGTATTCTCACAGGAGGATT30.11645962732919254No Hit
GTCCTGCACACTTTCCCAATAGAGCCTTCCTCCACTCCCCTGGGTGACTC30.11645962732919254No Hit
GCTAGCACCTGCTTCCAAGCCATGAGGTAATTGGGATTTATGCCTTTCTC30.11645962732919254No Hit
CAATGCATCCTGTGCAGCCATGGATGACTTTCAGCTGATCCCAATGATAA30.11645962732919254No Hit
CCCAGGAATATTTGAGAATTCATGCCTTGAAACAATGGAAGTTGTTCAAC30.11645962732919254No Hit
GCCACAACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCA30.11645962732919254No Hit
GTCTGGGCTCCCAGTAGGGGGCAATGAAAAGAAGGCCAAACTGGCAAATG30.11645962732919254No Hit
CTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCCCTCATGGT30.11645962732919254No Hit
GATTAGAGGTTTCGTATACTTTGTTGAAACTTTAGCTAGGAGCATTTGCG30.11645962732919254No Hit
GAAAATAAGAGTACATGAAGGGTATGAAGAATTCACAATGGTTGGGAGAA30.11645962732919254No Hit
ATGTTATACAACAAAATGGAATTTGAACCATTTCAGTCTCTTGTCCCTAA30.11645962732919254No Hit
TCATTACCCTAAGGTATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGA30.11645962732919254No Hit
ATGCACACATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCG30.11645962732919254No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG30.11645962732919254No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAA30.11645962732919254No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC30.11645962732919254No Hit
CTTAATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAG30.11645962732919254No Hit
GGACAGCAGACTCCACCCCAGATGTGCCTTCATCTGGATCTTCAGTCAAT30.11645962732919254No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTGAA100.006684254144.999983
GTGCCTG100.006684254144.999981
Produced by FastQC (version 0.11.5)