FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_G05_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_G05_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGATGCTCACACATTCACAGTCAATGACGAAAATACCATCCTACTC12.0408163265306123No Hit
CCATGGCCTCCACCATGCTAGAAATTCCCACCGGTCTCCTATATGAACTG12.0408163265306123No Hit
CTAATAGATGGCACAGCATCACTGAGTCCTGGGATGATGATGGGCATGTT12.0408163265306123No Hit
CAAGAATGGTTCAATTTTGGCGTTCACTTCTTTTGACATTTGGGAAAGCT12.0408163265306123No Hit
GTTCATTGATTCCATTACATCCTTTTAGAAATCAATTAGCCATCCTCACA12.0408163265306123No Hit
GCTATGGATTTGTGGCTAATTTTAGCATGGAGCTACCCAGCTTTGGAGTG12.0408163265306123No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA12.0408163265306123No Hit
ATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTC12.0408163265306123No Hit
CGCCCAAGACCGTCCTTCGCATGTTGAACATGCCCCGCCTAAGAACAGGC12.0408163265306123No Hit
AGCGTGGCACAGGGCCCTGCTTGGGAGCGTGGCACATGGAGCGTGGCACA12.0408163265306123No Hit
ATGCTATGGAAAAGGAAGCTGTAACCAGCCACACCTGACATTCAGCTGTC12.0408163265306123No Hit
AAAGAAGGGAGTGTACATAAATACGGCCTTGCTCAATGCATCCTGTGCAG12.0408163265306123No Hit
CTCATAGTGAATGCACCAAACCATGAGGGAATACAAGCAGGAGTGGACAG12.0408163265306123No Hit
CATCTTGCTGTGGCCCAGGAGCTGCTTGTTCTCTCACCCTGTGAGTGGGG12.0408163265306123No Hit
CCATGATCTGCCATGACATAAGTATGATGATGTAAAAAATTAAGTTGTTT12.0408163265306123No Hit
AGATTACAGGAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCCCCGAAC12.0408163265306123No Hit
TCCCTAGTCTTGCCATTTTGTTTGAGAACATTATGGGTGCCATGCTCAGG12.0408163265306123No Hit
ATTTTAACTTGATTTCTGAAGTGGACAGGGCCGAAGGTCCCATGTTTCAA12.0408163265306123No Hit
ATTCACTATGAGAGCAAAATCGTCGGATGATTGGAGCCCCTCCCACCAGT12.0408163265306123No Hit
CCCTTGGCCTCGATATAGAAACAGCCACTCTTGGTGGGAAACAAATCGTG12.0408163265306123No Hit
AGGAGGCACAAGATGTGAGAATGGAAGTTGTTGTCCCAAATTAAGTTGGC12.0408163265306123No Hit
AAATAGGAGACATGCTCTTGAGGACTGCGATAGGCCAAGTGTCGAGGCCC12.0408163265306123No Hit
GTTTGGAGGTTGCCCGTTAGCACTTCTTCTTCTTTCTTGACTGATGATCC12.0408163265306123No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC12.0408163265306123No Hit
CCATTACATCCTTTAAGAAATCTATTAGCCTTCCTGACTCATTAGCTGTT12.0408163265306123No Hit
ATTTCAGAGCATCCATCAGCAGGAACTTTGACCGCTGATGGCAAAGAGGC12.0408163265306123No Hit
ATTTGACACTGTCCAAATAATAAAACTTCTCCCCTATGCAGCTGACGCAC12.0408163265306123No Hit
AAGAAATGCAACAAGGAGATTTATAAAGTTTATATTAAGAGGGAGAGACG12.0408163265306123No Hit
ACATTACAGAGGAACTAGCTTTAAGCTATTGATGAGAGAAGAAAAAAATA12.0408163265306123No Hit
GTATGGGTTCATTATAAAAGGAAGGTCTCATTCGAGAAATGATACAGATG12.0408163265306123No Hit
GCACCATGCTAGAAATTGCAACCGGTCTGCTATATGAACTGCAAGGGAAA12.0408163265306123No Hit
GTTCAGTGATGCTTAATGCTGGGCCATATCTCTTGTCTTCTTTGCCCAAA12.0408163265306123No Hit
ATCAATCTCTTTATGACTGACAAAGGGATTCAGCGGAATACAAATTCTTA12.0408163265306123No Hit
TATCATGACACACAAAGGAATGGAAGAAACGGCCTATCGGCCCTGTACAC12.0408163265306123No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCTCACGCAAAGATGG12.0408163265306123No Hit
GGCCAAGGACGCGTCACTAAGCTATTAAACTGTAGCACTTGCCACTTGCA12.0408163265306123No Hit
GATGGATCAAGTAAGAGAAAGTCGAAAACCAGGAACCGCTGAGATTGTAG12.0408163265306123No Hit
CCTTAAACAGTATGACAGTGATGAGCCAGAGCCCAGATCTCTAGCAAGCT12.0408163265306123No Hit
GTCTTTAGATCGATTGGCCTAACAGATAAAGAGTGAGGAGGGCTAATAGA12.0408163265306123No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA12.0408163265306123No Hit
CACAAATACCAGCAGAAATGCTAGCAACCATTCACCTGAAGTACTTCAAT12.0408163265306123No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCACTAGACATATGCAAGGAA12.0408163265306123No Hit
GGGCAATGAAAAGAAGGCCAAACTGGCAAATGTTGTGAGAAAGATGATGA12.0408163265306123No Hit
GAACATGGGCCTAGCCACTTGGCCTATCGCAGTCCTCAAGAGCATGTCTC12.0408163265306123No Hit
GAATGTCTTCATGCATTTGATTCCATCATATAGTGGTATTCCCTCCCCCT12.0408163265306123No Hit
GTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATC12.0408163265306123No Hit
AAGAAGAGCAGCAGTGTCAGCAGACCCATTAGCATCTCTCTTGGAAATGT12.0408163265306123No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT12.0408163265306123No Hit
ATTTTTAAGGGTTAGAGATCAAAGAGGGAAAGTACTATTGTCTCCCGAAG12.0408163265306123No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)