FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_G04_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_G04_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCCAATCATAAGTCTGGCGACCTTGAGCTAGTTTATCTACCCTTGTT11.1363636363636365No Hit
AGGGGCGCCATGCTGAGGATGGTTCTGAACCTGTCGGTTTGATTGCTGGT11.1363636363636365No Hit
TATATGTGGACTTCCCTCCGTGTTACTCCAATTTCAATGAACCGGTTCTC11.1363636363636365No Hit
ATGGAAGACTTTGTGCGACAATGCTTCAATCCAATGATCGTCGAGCTTGC11.1363636363636365No Hit
GAACCGGAACAGGATACACCATGGACACAGTAAACAGAACACACCAATAA11.1363636363636365No Hit
TAACAAAAGAGAAGAAAGAAGAGCTCCAGGATTGTAAAATTGCTCCCTTG11.1363636363636365No Hit
ACCATCTTCTTGGTCATGTTGTCTCTTACTCTCCTTTTTCTTTGAAAGTG11.1363636363636365No Hit
CAAAGAACATGAAGAGAACAAGCCAATTGAAGTGGGCACTCGGTGAAAAT11.1363636363636365No Hit
CCTGGGAGAATACCATGGCCACAATTATTGCCTCAGCAATTGACTGCTCG11.1363636363636365No Hit
ACAGAGATTTCTTTCACAATCACTGGGGACAACACTAAGTGGAATGAAAA11.1363636363636365No Hit
GGTAGTGAGTATTGACCGATTTTTAAGGGTTAGAGATCAAAGAGGGAACG11.1363636363636365No Hit
GGCTGGCATATGTCTACGGCTGGTTCGTCAGCTGGATTCTGTCTAAGGAT11.1363636363636365No Hit
GCAAAACCCAGGGATCATTAATCAGGCACTCCTCGATTGGTTCATATAGC11.1363636363636365No Hit
AACTACTTCAGTGCATGTGTGAGGAAGGAGTTGAACCAAGATGCATTAAG11.1363636363636365No Hit
ATGGATTGGGTGATGCCCCATTCCTTGATCGGCTCCGCCGAGATCAAAAG11.1363636363636365No Hit
GATTGCCTGATGGGCCTCTTTGCCATCAGCGGTCAAAGTTCCTGCTGATG11.1363636363636365No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG11.1363636363636365No Hit
GGTGTGGAGGGTCTGGAGTAAAGCAATGAGCTGACAAAAGTATTGGTGTA11.1363636363636365No Hit
CTCCCTTCATTATGTATTCAGTAGCCCTGCACTGGGACACTTATGGACTA11.1363636363636365No Hit
TATGAAACTTGATACTCTTAAATCTTCAAATGCAGCAGAGTGGCATGCCA11.1363636363636365No Hit
CATGGTGTCTAGGGCCCGGATTGATGCCAGGGTCGACTTCGAGTCTGGAC11.1363636363636365No Hit
ATATGATGCAATCAAATGCATGAAGACATGCTTTGGCTGGAAAGAGCCTA11.1363636363636365No Hit
GGCTATTAGTATCAGATGGAGGACCAAACTTATACAATATACGGAATCTT11.1363636363636365No Hit
ATGTAGGACCAAAGAAGGAAGACGGAAAACAAACCTGTATGGGTTCATTA11.1363636363636365No Hit
GGCGATTTCTTGTCTTATAGTTAAAAGCCTAGTTATGATTATTGCCCTGC11.1363636363636365No Hit
GGTAATGCCAGCCCATGGTCCAGCCAAAAGCATGGAATATGATGCCGTTG11.1363636363636365No Hit
TTTCTGAACCACTCGGGTTGATTTCTGGTGATATATGTAATCATCGCCAG11.1363636363636365No Hit
AGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGT11.1363636363636365No Hit
ATGTTGCCCCGATTGAACATATCGCAAGCATGAGGAGGAACTATTTTACA11.1363636363636365No Hit
GCCCTTAGTAGTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTT11.1363636363636365No Hit
GCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCCTCCTCATGCTTGC11.1363636363636365No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT11.1363636363636365No Hit
GTGCCAAGGAGGCACAGGATGTGATTATGGAAGTTGTTTTCCCAAATGAA11.1363636363636365No Hit
AGGCACGATCGGGTTCGTTGCCTTTTAGTCCGAGAGCTCGAATACTCCCC11.1363636363636365No Hit
CTTCAATACTTAATTTCAGAGCATCCCTCAGCAGGACCTTTGACCGCTGA11.1363636363636365No Hit
ATATAGTGGTATTCCCTCCCCCGCGTGAGACCGCTCTTCCATAATTAATT11.1363636363636365No Hit
GAGAAAGATGATGACTAATTCACAAGACACAGAGATTTCTTTCACAATCA11.1363636363636365No Hit
CCTATATGTGTATCTGTAGTCTTTGATGAACAATTCAAGAGCCATCTGGG11.1363636363636365No Hit
CCCACTAACTTGCAGGTCCTGTAGAATCTGTCCACTCCTGCTTGTATTCC11.1363636363636365No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATTGCCCTCAGAAT11.1363636363636365No Hit
CTCCCCTCCCCTGGGTGACTCTCCGCTTGGCCATGTTTCCGATTTGTTTT11.1363636363636365No Hit
GAATATGGGGAAGATCCGAAAATCGAAACTAACAAGTTAGCTGCAATATG11.1363636363636365No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT11.1363636363636365No Hit
ATATAAAACTTATTTCGAAAAGGTCGAAAGGTTGAAACATGGTACCTTCG11.1363636363636365No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTG11.1363636363636365No Hit
GTATTCCGATCATTCCCATCACATTGTCGATGGATTCAATTCCCCAGTTC11.1363636363636365No Hit
GCTAATGGGTCTGCTGACACTGCTGCTCTTCTTACTATGTTTCTAGCAGC11.1363636363636365No Hit
TCATATAAGTTCTTCACATTTGAATCGTGGTAGTCCAAAGTTCTTTCATT11.1363636363636365No Hit
ACCTATATGTGTATCTGTAGTCTTTGATGAACAATTGAAGAGCCATTTGG11.1363636363636365No Hit
TTGTAGAATCTGGTGACCCGAATGCACTATTGAAGCACCGATTTGAGATA11.1363636363636365No Hit
GTACATCTGTTCATCCTCAAGAATTCCCCTTTGGCTTGTGTTGAGAATAG11.1363636363636365No Hit
ATGTAACACAACAGGGGTAGAGAAGCCTAAATTTCTTCCTGATTTGTATG11.1363636363636365No Hit
GATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGT11.1363636363636365No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA11.1363636363636365No Hit
GTGCAGGACCTTACTGGCAAAATCTGTATTACCCCGGCTCTATGCGTCTC11.1363636363636365No Hit
CTATTAGCCTTCCTGACTCATTAGCTGTTAGGCCATTAGATCTAAAGACT11.1363636363636365No Hit
AAAATGAAGAGAAGATCCCAAGGACAAAGAACATGAAGAGAACAAGCCAA11.1363636363636365No Hit
CATTTGGAAGTTTGTTTCATGTATTCGGATTTCCATTTCATCGACGAACG11.1363636363636365No Hit
GGAAAATTGCATCCTACTCTGTTCTGGTGGGGCAGCAGCAAAGGGGAGAA11.1363636363636365No Hit
TTCCTGAAGTCTGCTTAAAATGGGAGCTAATGGATGATGATTATCGGGGA11.1363636363636365No Hit
GCAATTTTACAATCCTGGAGCTCTTCTTTCTTCTCTTTTGTTATTGCCAG11.1363636363636365No Hit
GACAAAATAAAGTAAAATGAATAAGTTTCAACAACACAATCATCCAAAAA11.1363636363636365No Hit
GCTCGCCGTTGGCAAGAGTGCGCAGGGAATTGCTGCTTAAGGCTGGGCCA11.1363636363636365No Hit
GTGAATTGACTGATTCAAGCTGGATAGAACTTGATGAAATAGGAGAAGAT11.1363636363636365No Hit
CATAAATACGGCCTTGCTCAATGCATCCTGTGCAGCCATGGATGACTTTC11.1363636363636365No Hit
CTGTTTAAGGTCTCCAACATCTTTGCAGTCATCAAAGTCTACTTTTTCTG11.1363636363636365No Hit
CCCCCTGGGCAGGCAGAACCTGCTTCGTGCCTCAAGGGGCCGTGCTAACG11.1363636363636365No Hit
GGATTACAAAGTCTTCCCCGAATGGTGTTGGCCAATGCAGTTGCTGCCGG11.1363636363636365No Hit
CCTGAGGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTTCTAGA11.1363636363636365No Hit
GGGAGTAGATGAATACTCCAGCACGGAGAGAGTGGTAGTGAGTATTGACC11.1363636363636365No Hit
TACAGGAACTATGCGCAAGCTTGCCGACCAAAGTCTCCCACCGAACTTCC11.1363636363636365No Hit
CTCTTCCAGGACATACTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTC11.1363636363636365No Hit
ATCCTCAACCCTGTAATTGGGTATCTCATTGCCATCATCCACTTCATTCT11.1363636363636365No Hit
CGAATGGATTGCAGCACTTCTGGTACATCTGTTCATCCTCAAGAATTCCC11.1363636363636365No Hit
CCGCAGATTGCCGGATGGGCCTCTGTGCCATCAGCGGTCCAAGTGCCGGC11.1363636363636365No Hit
ATGGTAGTCTCACAGGAGGATTGCATGATCAAGGCAGTTAGGGTCGATCT11.1363636363636365No Hit
ATTCTACAGGACCTGCAAGTTAGTGGGAATCAACATGAGCAAAAAGAAGT11.1363636363636365No Hit
TGAGGACTGCGATAGGCCAAGTGTCGAGGCCCATGTTCCTATATGTGAGA11.1363636363636365No Hit
GGGCTATGCTCCCCCCAAGCCAAAGGGGACTTCGGGAGGAGGAACCTATG11.1363636363636365No Hit
TCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATAT11.1363636363636365No Hit
CAATGCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAGA11.1363636363636365No Hit
CCCTTCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTT11.1363636363636365No Hit
TGTTCACGCTCACCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTT11.1363636363636365No Hit
CCCCATGAGACAGGCAGAACTACCAATTACCGCATGGAGTGGCAGCCGGG11.1363636363636365No Hit
GTATTCTTGCCCCCACTTCATTTGGGAAAACAACTTCCATAATCACATCC11.1363636363636365No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC11.1363636363636365No Hit
TCTTTGGTCATCGTATTTAATGTCAGTGCTCTTATTAGATAGCCTCTCTT11.1363636363636365No Hit
AGTCTGGGGGTTCCGGGAACTTTGCGGGCGATGCGGCTCTCTGCCCTGAT11.1363636363636365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATTT50.0145.02
TTTCTTG50.0145.06
GGCGATT50.0145.01
GGCTGTA50.0145.0145
TTCTTGT50.0145.07
TCTTGTC50.0145.08
GATTTCT50.0145.04
CTTGTCT50.0145.09
ATTTCTT50.0145.05
CGATTTC50.0145.03
Produced by FastQC (version 0.11.5)