FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_G03_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_G03_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTTCTGGTTGCCTTAGGGACAAGAGACTGAAATGGTTCAAATTCCATT13.571428571428571No Hit
GTTCCAGGTTGTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGAT13.571428571428571No Hit
GTGTATCTGTAGTCTTTGAGGACAAATTTAAGAGCCATCTGGGCCGTTGC13.571428571428571No Hit
CCCTAGACCCCAGGGCCTCCACCATGCTATAAATTCCAACCGGTCTCCTA13.571428571428571No Hit
TGATTGTATAAAAAACTATGAAAGCAAAATATTCGAATGATATGAGCATA13.571428571428571No Hit
GACTGTGAATGTGAGAGGATCAGGGTTGAGGATACTGGTAAGAGGCAATT13.571428571428571No Hit
GTATTCAGTAGCCCTGCAGTGGGACACTTCTGCTGTAAAATAGTTCATCC13.571428571428571No Hit
CCATGAGGGAATACAAGCAGGAGTGGACAGATTCTACAGGCCCTGCAAGT13.571428571428571No Hit
CGCTTTCATTGAAGAATCCCACCCAGGAATATTTGAGAATTAATGCCTTG13.571428571428571No Hit
CTTACTATGTTTCTAGCATCTATAATCAAACTTTTGTCAACATCATCATT13.571428571428571No Hit
CATATGCAAGGCAGCAATAGGGTTGAGGATTAGCTCATCTTTCAGTTTTG13.571428571428571No Hit
GTCCCTAAGTGCCTGAACAATGAGAAGCAATTTTCTAGATTCAGCCGAAA13.571428571428571No Hit
AACAAAGGTAGGGCTATTAGTATAAGAAGCAGGACCAAACTTATACAATA13.571428571428571No Hit
CAAATAGCAGACCCAATGCTATATAGAACAGCGCGGATGGCCTGCAGTCT13.571428571428571No Hit
GACCCATTAGCATCTCTCTTGGAAATGTGCCACAGCACACAGATTGGAGG13.571428571428571No Hit
GATGATTGGAGCCCATCCCACCAGTATATTGTCTTGGTGTATTTCTTTTG13.571428571428571No Hit
CATTGAAGTACTTCAGGTCAATGCTTGCTAGCATTTCTGCTGGTATTTGT13.571428571428571No Hit
TTATATACCTTAGGGTAATGAACTGTAGTTGTTGGTGGGCCATTCCAATT13.571428571428571No Hit
CTTCTTCAAGGGTGTAGTCCGCTTGGGTTGCCAGCTCCTCTCCTGTGAAT13.571428571428571No Hit
TACTCCAGGAGGGGAAGTTAGGAATGATGATGTTGATCAAAGCTGGATTA13.571428571428571No Hit
GCCTTATTGAATTCATTTTGGACCCAGCTTGCTAGAGATCTGGGCTCTGG13.571428571428571No Hit
GTCAAATGTCCCAAGCACATCCCGCATTTGCTGGAACAGTGTCCTTACGA13.571428571428571No Hit
TTTCACTATAAGACAAGAAATGGCCAGTAGGAGTCTATGGGATTCCTTTC13.571428571428571No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT13.571428571428571No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA13.571428571428571No Hit
GGATCATCCTGCCCATGGCACCCCTAAAGTTCGCAATCAAAAGGGCCAGC13.571428571428571No Hit
AACAAATAACAGCTCTGATGTTAAATACGAAGACCACAAATTATGAGTCA13.571428571428571No Hit
CTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTTAG13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)