FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_G02_FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_G02_FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277
Sequences flagged as poor quality0
Sequence length151
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT60.4698512137823023No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA30.23492560689115116No Hit
ATCTGAGGGGGCGTGGTGTCGTCCTCAAGAATGGTTCAATTTTGGCGTTC20.15661707126076743No Hit
AGTCAGTGAAACGCAAGGAACTGAGAAGTTGACAATAACTTATTCGTCAT20.15661707126076743No Hit
GGTGATATATGTAATCATCGCCAGGAACATTCGAGGATTTTGATTTTCAT20.15661707126076743No Hit
GGAGAAGGAAGCTTCCCTCGGAGCAGGAAGCCTGATGTGGGACTTGATCC20.15661707126076743No Hit
GAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATGACCA20.15661707126076743No Hit
GGGCTCCCTTCCTCCCCTGTGGATTAACACGGGCTTCCAGTGACAGATGC20.15661707126076743No Hit
GCATGGTGGGGGTGATATTGGGAAGGGAGACACGTCAGATCCCCAGAGAG20.15661707126076743No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT20.15661707126076743No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG20.15661707126076743No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT20.15661707126076743No Hit
GCCCCTGGGTCCTGCTCCCTCTCCTGCCCTCTAGGCACCTGCCTGCGTTG20.15661707126076743No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC20.15661707126076743No Hit
GTTATGTTTATTCCTAGGTACTTGCCATCCCCTCTCCCCCAAAGACCAGA20.15661707126076743No Hit
CACTAGACCAAAAGCAGCTTCTGTGGTCACTGTTCCCATCCTGTTGTATA20.15661707126076743No Hit
CTATTGAAGCTGGACAATACTGCACTTTTGTTATTTCTACGGATGGCTCT20.15661707126076743No Hit
CTACTGGGAGCCCAGACTGTTCAAGCTTTTCGCAAATGCTCCTAGCTAAA20.15661707126076743No Hit
ACAACAGTCAGTGTGCAAATCAGTGTTCTGGTCTGTTCTGGTTTTGCATG20.15661707126076743No Hit
GTCCTAATGCCTCTCCTCCCGACTGTTCGTGCTGGTTTCCCAGACACCAT20.15661707126076743No Hit
CTATATATGCATGGGGGTTCTTGAAGGAAAATGCAGCTCAGGGAGACAGT20.15661707126076743No Hit
ACTTAGGACTGGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAAT20.15661707126076743No Hit
ATGTGGGCCAGTGCCCATGGAGGAGGGCAAGGGTTAGGTCCAAGTGGAAT20.15661707126076743No Hit
GCACATGTCTGTATCAGCAGAGTTCACAAATACATTGAAAGTGGCTTTTT20.15661707126076743No Hit
GCCCAGGTCAGGCAGGCTGGGAGAATGCTGGCTGTACGGAAAGGCTGGAG20.15661707126076743No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTCC100.0062713427144.999981
Produced by FastQC (version 0.11.5)