FastQCFastQC Report
Fri 2 Oct 2020
HTGTLBGXG_n01_S2_R2_G02-FD.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTGTLBGXG_n01_S2_R2_G02-FD.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106509
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT38820.3508331156818426No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA33010.29832563494738856No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT30030.27139408716964797No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA24870.22476093732631186No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACCTCTTAACAATA24030.21716949432855948No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC17640.15942030295280019No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG17530.15842618541738024No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC14990.13547110778131943No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT14960.135199984817114No Hit
TACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCTGTGGCA14110.1275181674979598No Hit
AAATAAGACAGGGACATTTGAATTCACAAGCTTTTTTTATCGCTATGGAT14020.12670479860534348No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA13600.12290907710646728No Hit
GTATAAGTTTGGTCCTCCATCTGATACTAATAGCCCTACCTTTGATTGGG11910.10763581678956069No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG11680.10555720739731896No Hit
CCTTGGAGAAGGACCTGCATGGTGGGGGTGATATTGGGAAGGGAGACACG11590.10474383850470263No Hit
GTATATTGTCTTGGTGTATTTCTTTTGTCCAAGATTCAGTATCGAGACTC11230.10149036293423731No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGT406.6581626E-572.51173145
AGCGAAA4650.063.935071
CGAAAGC5050.058.868253
ATATTCA1700.051.1824384
CAAGGTA1004.7062713E-850.746749
CTATAGG1950.048.3411561
GCGAAAG6250.047.5655442
GGTCTAC657.3310774E-444.6226041
ACATCGA1307.355993E-944.6125228
TACATAC12100.044.3440136
GACATCG1351.0268195E-842.9602057
TACATCA10100.040.9206125
GTATGTA3250.040.1512689
GTATTAC10250.039.6161651
GGTCGTT1651.36788E-939.5429157
GTTCGGA750.001482275538.672924145
CATACAT13900.038.5946438
CACGGAC951.02825565E-438.1623464
ACATACA14150.037.912767
ATACATG2900.037.5043686
Produced by FastQC (version 0.11.5)